NSC-38270 Exhibits Anti-invasive and Pro-apoptotic Effects on Hepatocellular Carcinoma Huh7 Cells.

IF 1.6 4区医学 Q4 ONCOLOGY Anticancer research Pub Date : 2024-09-01 DOI:10.21873/anticanres.17212

Jihye Seo, Minseo Park, Sayeon Cho

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Abstract

Background/aim: Hepatocellular carcinoma (HCC) is a main type of liver cancer with high metastatic potential, and its incidence is steadily increasing worldwide. However, the development of new drugs for the treatment of HCC is still insufficient. This study aimed to determine the anticancer effect of NSC-38270, a natural product, on HCC.

Materials and methods: After treating HCC Huh7 cells with NSC-38270, cell growth, wound healing, migration, and invasion assays were conducted. We investigated the effects of NSC-38270 on Twist1, a crucial epithelial-mesenchymal transition (EMT)-related transcription factor. In addition, apoptosis, histone H2A.X activation, and cell morphology assays were performed in Huh7 and immortalized normal liver cells following treatment with NSC-38270.

Results: NSC-38270 reduced the migration and invasion ability of Huh7 cells, accompanied by a decrease in Twist1. Furthermore, NSC-38270 induced apoptosis in Huh7 cells, whereas apoptosis was not observed in immortalized normal liver cells (THLE-2 cells and Chang liver cells).

Conclusion: NSC-38270 exhibited significant inhibitory effects on the migration and invasion of Huh7 cells by repressing Twist1. Importantly, it induced cancer cell-specific apoptotic effects. These findings suggest that NSC-38270 holds promising potential as a therapeutic candidate for cancer treatment.

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NSC-38270 对肝细胞癌 Huh7 细胞具有抗侵袭和促凋亡作用

背景/目的：肝细胞癌（HCC）是肝癌的一种主要类型，具有高度转移性，其发病率在全球范围内稳步上升。然而，治疗 HCC 的新药开发仍然不足。本研究旨在确定天然产物 NSC-38270 对 HCC 的抗癌作用：用 NSC-38270 处理 HCC Huh7 细胞后，进行了细胞生长、伤口愈合、迁移和侵袭试验。我们研究了 NSC-38270 对 Twist1 的影响，Twist1 是一种关键的上皮-间质转化（EMT）相关转录因子。此外，在使用 NSC-38270 处理 Huh7 和永生化正常肝细胞后，还进行了细胞凋亡、组蛋白 H2A.X 活化和细胞形态学检测：结果：NSC-38270降低了Huh7细胞的迁移和侵袭能力，同时降低了Twist1。此外，NSC-38270 还诱导 Huh7 细胞凋亡，而在永生化正常肝细胞（THLE-2 细胞和 Chang 肝细胞）中未观察到凋亡：结论：NSC-38270通过抑制Twist1对Huh7细胞的迁移和侵袭有明显的抑制作用。重要的是，它还能诱导癌细胞特异性凋亡。这些研究结果表明，NSC-38270 具有作为癌症治疗候选药物的潜力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Anticancer research 医学-肿瘤学

CiteScore

3.70

自引率

10.00%

发文量

566

审稿时长

2 months

期刊介绍： ANTICANCER RESEARCH is an independent international peer-reviewed journal devoted to the rapid publication of high quality original articles and reviews on all aspects of experimental and clinical oncology. Prompt evaluation of all submitted articles in confidence and rapid publication within 1-2 months of acceptance are guaranteed. ANTICANCER RESEARCH was established in 1981 and is published monthly (bimonthly until the end of 2008). Each annual volume contains twelve issues and index. Each issue may be divided into three parts (A: Reviews, B: Experimental studies, and C: Clinical and Epidemiological studies). Special issues, presenting the proceedings of meetings or groups of papers on topics of significant progress, will also be included in each volume. There is no limitation to the number of pages per issue.