Escherichia coli novel sequence type 11873 harbours a new CTX-M-15–carrying multidrug resistance type 1/2 hybrid IncC plasmid

IF 3.7 3区 医学 Q2 INFECTIOUS DISEASES Journal of global antimicrobial resistance Pub Date : 2024-08-26 DOI:10.1016/j.jgar.2024.08.008
Didrik H. Grevskott , Francisco Salvà-Serra , Edward R.B. Moore , Nachiket P. Marathe
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Abstract

Objective

The aim of the current study was to determine the genomic map of the resistance genes of two CTX-M-15–carrying Escherichia coli strains belonging to novel sequence type (ST) 11873. Complete, closed genome sequences of the E. coli strains were obtained by applying a combination of short-read Illumina and long-read Oxford Nanopore-based sequencing.

Methods

Isolation of E. coli was performed using ECC CHROMagar and antibiotic sensitivity patterns were determined using Sensititre EUVSEC plates. Whole-genome sequencing was performed for two E. coli strains (3–338 and 5–325) using Illumina MiSeq- and Oxford Nanopore MinION-based sequencing.

Results

The complete genome of strain 3–338 (GenBank accession no. CP130007–17) was assembled into a circular chromosome of 4.65 Mb and 10 plasmids (between 2 and 148 kb). Strain 5–325 (CP130018–27) exhibited a circular chromosome of 4.7 Mb and 9 plasmids (between 2 and 148 kb). Both strains carried an identical type 1/2 hybrid IncC plasmid (∼148 kb) harbouring multiple antibiotic resistance genes (ARGs), including blaCTX−M-15, blaOXA-1, blaTEM-1, qnrS1, sul2, aphA1, aacC2, mph(A) and floR. This plasmid also carried heavy metal resistance genes, such as chrA and arsR. Strain 5–325 carried an additional IncFIB plasmid (∼78 kb) harbouring additional ARGs, including blaTEM-1, qnrS1, tet(A), dfrA14, sul2, strA and strB.

Conclusions

Our study shows the emergence of a CTX-M-15–carrying type 1/2 hybrid IncC plasmid in novel E. coli ST11873. These findings emphasise the need for population-based sewage surveillance for understanding the prevalence of antibiotic resistance in pathogens in order to mitigate the further spread of such resistance factors.
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11873型大肠埃希菌新序列中携带一种新的CTX-M-15携带多重耐药1/2型杂交IncC质粒。
研究目的本研究旨在确定属于新型序列类型(ST)11873 的两株携带 CTX-M-15 的大肠埃希菌耐药基因的基因组图谱。大肠杆菌菌株的完整闭合基因组序列是通过基于短读程 Illumina 和长读程 Oxford Nanopore 的测序组合获得的:使用 ECC CHROMagar™ 进行大肠杆菌分离,并使用 Sensititre™ EUVSEC 平板确定抗生素敏感性模式。使用基于 Illumina MiSeq 和 Oxford Nanopore MinION 的测序技术对两株大肠杆菌(3-338 和 5-325)进行了全基因组测序:结果:菌株 3-338 的完整基因组(GenBank 编号:CP130007-17)被组装成一个 4.65 Mb 的环状染色体和 10 个质粒(2 kb 至 148 kb)。菌株 5-325(CP130018-27)的环状染色体为 4.7 Mb,有 9 个质粒(介于 2 kb 和 148 kb 之间)。两株菌株都携带有一个相同的 1/2 型杂交 IncC 质粒(148 kb),其中含有多个抗生素抗性基因(ARGs),包括 blaCTX-M-15、blaOXA-1、blaTEM-1、qnrS1、sul2、aphA1、aacC2、mph(A) 和 floR。该质粒还携带重金属抗性基因,如 chrA 和 arsR。菌株 5-325 还携带一个额外的 IncFIB 质粒(∼78 kb),该质粒还携带其他 ARGs,包括 blaTEM-1、qnrS1、tet(A)、dfrA14、sul2、strA 和 strB:我们的研究表明,在新型大肠杆菌 ST11873 中出现了携带 CTX-M-15 的 1/2 型混合 IncC 质粒。这些发现强调了以人群为基础进行污水监测的必要性,以了解病原体中抗生素耐药性的流行情况,从而减少此类耐药性因素的进一步扩散。
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来源期刊
Journal of global antimicrobial resistance
Journal of global antimicrobial resistance INFECTIOUS DISEASES-PHARMACOLOGY & PHARMACY
CiteScore
8.70
自引率
2.20%
发文量
285
审稿时长
34 weeks
期刊介绍: The Journal of Global Antimicrobial Resistance (JGAR) is a quarterly online journal run by an international Editorial Board that focuses on the global spread of antibiotic-resistant microbes. JGAR is a dedicated journal for all professionals working in research, health care, the environment and animal infection control, aiming to track the resistance threat worldwide and provides a single voice devoted to antimicrobial resistance (AMR). Featuring peer-reviewed and up to date research articles, reviews, short notes and hot topics JGAR covers the key topics related to antibacterial, antiviral, antifungal and antiparasitic resistance.
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