Development of an AIE-active fluorescent probe for the simultaneous detection of Al3+ and viscosity and imaging in Alzheimer’s disease model

IF 4.5 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY Bioorganic Chemistry Pub Date : 2024-08-28 DOI:10.1016/j.bioorg.2024.107768

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Abstract

Alzheimer’s disease is associated both with imbalances in Al³⁺ production and changes in viscosity in cells. Their simultaneous measurement could therefore provide valuable insights into Alzheimer’s disease pathology. Their simultaneous measurement would therefore be of great value in investigating the pathological mechanism of Alzheimer’s disease. We designed a fluorescent probe YM2T with AIE effect that is capable of selectively responding to Al³⁺ by fluorescence colormetrics and to viscosity by fluorescence “turn on” modes. Additionally, Al³⁺ and viscosity were simultaneously detected in PC12 cells using the low cytotoxic probe YM2T via blue and green fluorescence channels. More importantly, the YM2T probe was used to image mice with AD. Hence, the YM2T probe shows potential as a useful molecular instrument for studying the pathological impact of Al³⁺ and viscosity.

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开发一种 AIE 活性荧光探针，用于同时检测 Al3+、粘度和阿尔茨海默病模型成像

阿尔茨海默病与 Al3+ 生成失衡和细胞粘度变化有关。因此，同时测量这两种物质可以为了解阿尔茨海默病的病理提供有价值的信息。因此，同时测量它们对研究阿尔茨海默病的病理机制具有重要价值。我们设计了一种具有 AIE 效应的荧光探针 YM2T，它能够通过荧光色谱法对 Al3+做出选择性反应，并通过荧光 "开启 "模式对粘度做出选择性反应。此外，使用低细胞毒性探针 YM2T，可通过蓝色和绿色荧光通道同时检测 PC12 细胞中的 Al3+和粘度。更重要的是，YM2T 探针被用于对患有注意力缺失症的小鼠进行成像。因此，YM2T探针有望成为研究Al3+和粘度对病理影响的有用分子仪器。

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来源期刊

Bioorganic Chemistry 生物-生化与分子生物学

CiteScore

9.70

自引率

3.90%

发文量

679

审稿时长

31 days

期刊介绍： Bioorganic Chemistry publishes research that addresses biological questions at the molecular level, using organic chemistry and principles of physical organic chemistry. The scope of the journal covers a range of topics at the organic chemistry-biology interface, including: enzyme catalysis, biotransformation and enzyme inhibition; nucleic acids chemistry; medicinal chemistry; natural product chemistry, natural product synthesis and natural product biosynthesis; antimicrobial agents; lipid and peptide chemistry; biophysical chemistry; biological probes; bio-orthogonal chemistry and biomimetic chemistry. For manuscripts dealing with synthetic bioactive compounds, the Journal requires that the molecular target of the compounds described must be known, and must be demonstrated experimentally in the manuscript. For studies involving natural products, if the molecular target is unknown, some data beyond simple cell-based toxicity studies to provide insight into the mechanism of action is required. Studies supported by molecular docking are welcome, but must be supported by experimental data. The Journal does not consider manuscripts that are purely theoretical or computational in nature. The Journal publishes regular articles, short communications and reviews. Reviews are normally invited by Editors or Editorial Board members. Authors of unsolicited reviews should first contact an Editor or Editorial Board member to determine whether the proposed article is within the scope of the Journal.