The role of M1 (CD11c) and M2 (CD163) interplay in the pathogenesis of oral submucous fibrosis and its malignant transformation: An immunohistochemical analysis

IF 3.7 3区医学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Cytokine Pub Date : 2024-08-31 DOI:10.1016/j.cyto.2024.156742

Nishreen M. Parekh, Rajiv S. Desai, Shivani P. Bansal, Pankaj M. Shirsat, Pooja S. Prasad

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Abstract

Objectives

The M1/M2 macrophage framework is crucial in organ fibrosis and its progression to malignancy. This study investigated the possible role of M1/M2 macrophage interplay in the pathogenesis of oral submucous fibrosis (OSF) and its malignant transformation by analysing immunohistochemical expression of CD11c (M1) and CD163 (M2) markers.

Methods

Immunohistochemistry was performed using primary antibodies against CD11c and CD163 on ten formalin-fixed paraffin-embedded tissue blocks for each group: (i) Stage 1 OSF, (ii) Stage 2 OSF, (iii) Stage 3 OSF, (iv) Stage 4 OSF, (v) well-differentiated squamous cell carcinoma (WDSCC) with OSF, and (vi) WDSCC without OSF. Ten cases of healthy buccal mucosa (NOM) served as controls.

Results

Epithelial quick scores of M1 (CD11c) in NOM, Stages 1–4 OSF, and WDSCC with and without OSF were 0, 1.8, 2.9, 0.4, 0, 0, and 0, while connective tissue scores were 0, 3.2, 4.3, 2.7, 0.5, 1.2, and 2.4, respectively. Epithelial scores for M2 (CD163) were 0, 0.8, 0.8, 2.1, 0.6, 0.8, and 0.2, and connective tissue scores were 0, 1.8, 2.6, 3.9, 2.2, 5, and 4.4, respectively. Stages 3 and 4 OSF, WDSCC with and without OSF exhibited higher M2/M1 ratios compared to NOM and Stages 1–2 OSF.

Conclusion

The interaction between M1 (CD11c) and M2 (CD163) macrophages, leading to M2 polarisation, plays a crucial role in the pathogenesis of OSF and its potential malignant transformation.

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M1（CD11c）和 M2（CD163）在口腔黏膜下纤维化及其恶性转化的发病机制中的作用：免疫组化分析

目的M1/M2巨噬细胞框架在器官纤维化及其恶变过程中至关重要。本研究通过分析 CD11c（M1）和 CD163（M2）标记物的免疫组化表达，探讨 M1/M2 巨噬细胞相互作用在口腔黏膜下纤维化（OSF）及其恶性转化的发病机制中可能发挥的作用。方法使用针对 CD11c 和 CD163 的一抗对每组 10 个福尔马林固定石蜡包埋组织块进行免疫组化：(i) 1 期 OSF；(ii) 2 期 OSF；(iii) 3 期 OSF；(iv) 4 期 OSF；(v) 伴有 OSF 的分化良好鳞状细胞癌（WDSCC）；(vi) 不伴有 OSF 的分化良好鳞状细胞癌。结果NOM、1-4期OSF、有OSF和无OSF的WDSCC的M1（CD11c）上皮快速评分分别为0、1.8、2.9、0.4、0、0和0，而结缔组织评分分别为0、3.2、4.3、2.7、0.5、1.2和2.4。M2（CD163）的上皮评分分别为 0、0.8、0.8、2.1、0.6、0.8 和 0.2，结缔组织评分分别为 0、1.8、2.6、3.9、2.2、5 和 4.4。结论 M1（CD11c）和M2（CD163）巨噬细胞之间的相互作用导致M2极化，在OSF的发病机制及其潜在的恶性转化中起着至关重要的作用。

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来源期刊

Cytokine 医学-免疫学

CiteScore

7.60

自引率

2.60%

发文量

262

审稿时长

48 days

期刊介绍： The journal Cytokine has an open access mirror journal Cytokine: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. * Devoted exclusively to the study of the molecular biology, genetics, biochemistry, immunology, genome-wide association studies, pathobiology, diagnostic and clinical applications of all known interleukins, hematopoietic factors, growth factors, cytotoxins, interferons, new cytokines, and chemokines, Cytokine provides comprehensive coverage of cytokines and their mechanisms of actions, 12 times a year by publishing original high quality refereed scientific papers from prominent investigators in both the academic and industrial sectors. We will publish 3 major types of manuscripts: 1) Original manuscripts describing research results. 2) Basic and clinical reviews describing cytokine actions and regulation. 3) Short commentaries/perspectives on recently published aspects of cytokines, pathogenesis and clinical results.