ESIPT+TICT-based Near-IR Detection of Al3+ and F- in Live Cells: Molecular Docking with Acetylcholinesterase.

IF 3.5 3区化学 Q2 CHEMISTRY, MULTIDISCIPLINARY Chemistry - An Asian Journal Pub Date : 2024-09-01 DOI:10.1002/asia.202400678

Sanjeev Kumar, Sheikh Showkat, Satinder Kaur, Satwinderjeet Kaur, Prakash Chander Mishra, Prabhpreet Singh

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Abstract

The interplay of ESIPT+TICT mechanisms in 1,8-naphthalimide-hydroxyquinoline (NQ-OH) molecular rotor were reported for the near-IR 'turn-on' emission (λmax 600 nm) and ratiometric (A405nm/A345nm) absorbance-based detection of Al3+ ions in aqueous medium and live cells which were supported by NMR, IR and CV techniques. The limit of detection (LOD) for Al3+ ions is 100 nM and 14.57 nM. The self-assembled spherical aggregates of NQ-OH transformed into cuboidal aggregates upon coordination with Al3+ ions supported by microscopic and dynamic light scattering (DLS) techniques. The complex NQ-OH+Al3+ was further used for the secondary detection of F- ions in aqueous medium via displacement approach with LOD as low as 2.67 nM. A deeper study revealed that the NQ-OH is a solvatochromic dye. Probably, the NQ-OH either in the aggregated state or in the coordination state with Al3+ ions, showed an increase in the emission intensity at 600 nm due to inhibition of the ESIPT process and trigger of the TICT process. We have demonstrated the utility of NQ-OH for the detection of Al3+ ions and NQ-OH+Al3+ complex for the detection of F- ions in MCF7 live cells. We have also discussed the molecular docking studies of NQ-OH with acetylcholinesterase enzyme.

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基于 ESIPT+TICT 的活细胞中 Al3+ 和 F- 的近红外检测：与乙酰胆碱酯酶的分子对接。

报告了 1,8-萘二甲酰亚胺-羟基喹啉（NQ-OH）分子转子中 ESIPT+TICT 机制的相互作用，用于水介质和活细胞中 Al3+ 离子的近红外 "开启 "发射（λmax 600 nm）和比吸收（A405nm/A345nm）检测，并得到了 NMR、IR 和 CV 技术的支持。Al3+ 离子的检测限（LOD）分别为 100 nM 和 14.57 nM。通过显微镜和动态光散射（DLS）技术，NQ-OH 的自组装球形聚集体在与 Al3+ 离子配位后转变为立方体聚集体。NQ-OH+Al3+ 复合物被进一步用于通过置换法二次检测水介质中的 F- 离子，其 LOD 低至 2.67 nM。深入研究发现，NQ-OH 是一种溶解变色染料。可能是由于抑制了 ESIPT 过程并触发了 TICT 过程，NQ-OH 在聚集态或与 Al3+ 离子配位态时，在 600 纳米波长处的发射强度都会增加。我们证明了 NQ-OH 在 MCF7 活细胞中检测 Al3+ 离子和 NQ-OH+Al3+ 复合物检测 F- 离子的实用性。我们还讨论了 NQ-OH 与乙酰胆碱酯酶的分子对接研究。

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来源期刊

Chemistry - An Asian Journal 化学-化学综合

CiteScore

7.00

自引率

2.40%

发文量

535

审稿时长

1.3 months

期刊介绍： Chemistry—An Asian Journal is an international high-impact journal for chemistry in its broadest sense. The journal covers all aspects of chemistry from biochemistry through organic and inorganic chemistry to physical chemistry, including interdisciplinary topics. Chemistry—An Asian Journal publishes Full Papers, Communications, and Focus Reviews. A professional editorial team headed by Dr. Theresa Kueckmann and an Editorial Board (headed by Professor Susumu Kitagawa) ensure the highest quality of the peer-review process, the contents and the production of the journal. Chemistry—An Asian Journal is published on behalf of the Asian Chemical Editorial Society (ACES), an association of numerous Asian chemical societies, and supported by the Gesellschaft Deutscher Chemiker (GDCh, German Chemical Society), ChemPubSoc Europe, and the Federation of Asian Chemical Societies (FACS).