Genetic diversity of Bartonella spp. among cave-dwelling bats from Colombia

IF 2.1 3区 医学 Q2 PARASITOLOGY Acta tropica Pub Date : 2024-08-29 DOI:10.1016/j.actatropica.2024.107370
Carlos Ramiro Silva-Ramos , Jesús A. Ballesteros-Ballesteros , Sandra M. Chala-Quintero , J. Manuel Matiz-González , María T. Herrera-Sepúlveda , Álvaro A Faccini-Martínez , Adriana del Pilar Pulido-Villamarín , Marylin Hidalgo , C. Miguel Pinto , Jairo Pérez-Torres , Claudia Cuervo
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Abstract

Bartonella is a bacterial genus that comprises arthropod-borne microorganisms. Several Bartonella isolates have been detected from bats worldwide, which are thought to be undescribed species. We aimed to test the presence of Bartonella spp. among bats from Colombia, and evaluate the genetic diversity of bat-associated Bartonella spp. through phylogenetic analyses.

A total of 108 bat blood samples were collected from three bat species (Carollia perspicillata, Mormoops megalophylla, and Natalus tumidirostris) that inhabit the Macaregua cave. The Bartonella ssrA gene was targeted through real-time and end-point PCR; additionally, the gltA and rpoB genes were detected by end-point PCR. All obtained amplicons were purified and bidirectionally sequenced for phylogenetic analysis using a concatenated supermatrix and a supertree approaches.

A detection frequency of 49.1 % (53/108) for Bartonella spp. was evidenced among bat blood samples, of which 59.1 % (26/44), 54.3 % (19/35) and 27.6 % (8/29) were identified in Carollia perspicillata, Natalus tumidirostris and Mormoops megalophylla respectively. A total of 35 ssrA, 5 gltA and 4 rpoB good-quality sequences were obtained which were used for phylogenetic analysis. All obtained bat sequences clustered together with sequences obtained from Neotropical bat species into two bat-restricted clades namely clade A and clade N.

We detected the presence of Bartonella spp. that clustered within two different bat-associated Bartonella clades, giving the first data of the genetic diversity of these bacteria among bats from Colombia.

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哥伦比亚穴居蝙蝠中巴顿氏菌属的遗传多样性。
巴顿氏菌是一种由节肢动物传播的细菌属微生物。全球已从蝙蝠身上检测到多个巴顿氏菌分离株,这些分离株被认为是未被描述的物种。我们的目的是检测哥伦比亚蝙蝠中是否存在巴顿氏菌属,并通过系统发育分析评估蝙蝠相关巴顿氏菌属的遗传多样性。研究人员从栖息在 Macaregua 洞穴中的三种蝙蝠(Carollia perspicillata、Mormoops megalophylla 和 Natalus tumidirostris)身上采集了 108 份蝙蝠血液样本。通过实时和终点聚合酶链式反应检测了巴顿氏菌 ssrA 基因;此外,还通过终点聚合酶链式反应检测了 gltA 和 rpoB 基因。对所有获得的扩增子进行了纯化和双向测序,并使用超级矩阵和超级树方法进行了系统进化分析。在蝙蝠血液样本中,巴顿氏杆菌的检出率为 49.1%(53/108),其中在 Carollia perspicillata、Natalus tumidirostris 和 Mormoops megalophylla 中的检出率分别为 59.1%(26/44)、54.3%(19/35)和 27.6%(8/29)。共获得 35 条 ssrA、5 条 gltA 和 4 条 rpoB 的高质量序列,并将其用于系统进化分析。所有获得的蝙蝠序列与从新热带蝙蝠物种中获得的序列聚类为两个蝙蝠限制支系,即支系A和支系N。我们检测到巴顿菌属的存在,它们聚类在两个不同的蝙蝠相关巴顿菌支系中,首次提供了哥伦比亚蝙蝠中这些细菌遗传多样性的数据。
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来源期刊
Acta tropica
Acta tropica 医学-寄生虫学
CiteScore
5.40
自引率
11.10%
发文量
383
审稿时长
37 days
期刊介绍: Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.
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