Identification and antifungal sensitivity of Fusarium species isolated from piscine hosts.

IF 1.1 4区 农林科学 Q3 FISHERIES Diseases of aquatic organisms Pub Date : 2024-08-29 DOI:10.3354/dao03809
Victoria Chanu Khangembam, Dimpal Thakuria, Ritesh Shantilal Tandel, Vinita Pant, Nityanand Pandey, Pramod Kumar Pandey
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Abstract

Fusarium is a huge genus of filamentous fungi that has the potential to cause emerging diseases. Members of this genus can cause infections in plants, animals and humans. Here, we report the isolation of F. oxysporum and F. equiseti from 2 important fish species, Oncorhynchus mykiss (rainbow trout) and Tor putitora (golden mahseer), respectively. F. oxysporum has emerged as a significant fungal pathogen causing infection in many fish. However, F. equiseti has been isolated mainly from plants. As far as the available literatures are concerned, this is the first report on the isolation of F. oxysporum and F. equiseti from these hosts. The isolates were identified based on growth morphology and microscopic observation. F. oxysporum produced violet pigmentation on potato dextrose agar, while F. equiseti had yellow colouration. F. oxysporum produced 1- to 2-celled microconidia along with straight or curved macroconidia having 3 to 4 septa. F. equiseti produced abundant macroconidia with 4 or more septa. Species were further confirmed based on the nucleotide sequences of the internal transcribed spacer region. In a molecular phylogeny analysis, F. oxysporum and F. equiseti formed 2 different clades. In an antifungal sensitivity assay, F. oxysporum was found to be susceptible to clotrimazole with a minimum inhibitory concentration of 1.0 µg ml-1, whereas F. equiseti was susceptible to clotrimazole, ketoconazole and fluconazole. Overall, the main findings of this study are the infection of new hosts by Fusarium species and the limited activity of many antifungal drugs against these pathogens.

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从鱼类宿主中分离出的镰刀菌种的鉴定和抗真菌敏感性。
镰刀菌是一个庞大的丝状真菌属,有可能导致新出现的疾病。该属的成员可导致植物、动物和人类感染。在此,我们报告了分别从两种重要鱼类 Oncorhynchus mykiss(虹鳟鱼)和 Tor putitora(金鳟鱼)中分离出 F. oxysporum 和 F. equiseti 的情况。F. oxysporum 已成为导致许多鱼类感染的重要真菌病原体。然而,F. equiseti 主要是从植物中分离出来的。就现有文献而言,这是首次从这些寄主中分离出 F. oxysporum 和 F. equiseti 的报告。根据生长形态和显微镜观察对分离物进行了鉴定。F. oxysporum 在马铃薯葡萄糖琼脂上产生紫色色素,而 F. equiseti 则呈黄色。F. oxysporum 产生 1 至 2 室的微菌丝,以及有 3 至 4 个隔膜的直或弯曲的大菌丝。F. equiseti 产生大量具有 4 个或更多隔膜的大锥体。根据内部转录间隔区的核苷酸序列进一步确认了物种。在分子系统进化分析中,F. oxysporum 和 F. equiseti 形成了两个不同的支系。在抗真菌敏感性试验中,发现 F. oxysporum 对克霉唑敏感,最低抑菌浓度为 1.0 µg ml-1,而 F. equiseti 对克霉唑、酮康唑和氟康唑敏感。总之,这项研究的主要发现是镰刀菌感染了新的寄主,而许多抗真菌药物对这些病原体的活性有限。
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来源期刊
Diseases of aquatic organisms
Diseases of aquatic organisms 农林科学-兽医学
CiteScore
3.10
自引率
0.00%
发文量
53
审稿时长
8-16 weeks
期刊介绍: DAO publishes Research Articles, Reviews, and Notes, as well as Comments/Reply Comments (for details see DAO 48:161), Theme Sections and Opinion Pieces. For details consult the Guidelines for Authors. Papers may cover all forms of life - animals, plants and microorganisms - in marine, limnetic and brackish habitats. DAO''s scope includes any research focusing on diseases in aquatic organisms, specifically: -Diseases caused by coexisting organisms, e.g. viruses, bacteria, fungi, protistans, metazoans; characterization of pathogens -Diseases caused by abiotic factors (critical intensities of environmental properties, including pollution)- Diseases due to internal circumstances (innate, idiopathic, genetic)- Diseases due to proliferative disorders (neoplasms)- Disease diagnosis, treatment and prevention- Molecular aspects of diseases- Nutritional disorders- Stress and physical injuries- Epidemiology/epizootiology- Parasitology- Toxicology- Diseases of aquatic organisms affecting human health and well-being (with the focus on the aquatic organism)- Diseases as indicators of humanity''s detrimental impact on nature- Genomics, proteomics and metabolomics of disease- Immunology and disease prevention- Animal welfare- Zoonosis
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