Tamara Juárez-Velázquez , José Arnold González-Garrido , Irma Sánchez-Lombardo , Nelly del Carmen Jiménez-Pérez , Ivonne María Olivares-Corichi , José Rubén García-Sánchez , Oswaldo Hernández-Abreu
{"title":"Untargeted metabolic analysis of Epaltes mexicana by LC-QTOF-MS: Terpenes with activity against human cancer cell lines","authors":"Tamara Juárez-Velázquez , José Arnold González-Garrido , Irma Sánchez-Lombardo , Nelly del Carmen Jiménez-Pérez , Ivonne María Olivares-Corichi , José Rubén García-Sánchez , Oswaldo Hernández-Abreu","doi":"10.1016/j.fitote.2024.106194","DOIUrl":null,"url":null,"abstract":"<div><p><em>Epaltes mexicana</em> is a plant widely used in traditional medicine and as a food in Mexico; however, its phytochemical and pharmacological studies are limited. This study aimed to identify the active secondary metabolites of <em>Epaltes mexicana</em> and determine its cytotoxic activity on cancer cell lines. Three organic extracts were obtained by maceration using <em>n</em>-hexane, dichloromethane, and methanol. The <em>n</em>-hexane extract was fractioned by simple column chromatography. Eight terpenes were annotated in collection 6 (<strong>C6</strong>) by LC-QTOF-MS using a gradient elution and Electrospray Ionization (ESI) in positive ion mode: <strong>1)</strong> Gibberellin A15, <strong>2)</strong> farfugin A, <strong>3)</strong> dehydromyodesmone, <strong>4)</strong> eremopetasitenin A1, <strong>5)</strong> hydroxyisonobilin, <strong>6)</strong> anhydrocinnzeylanine, <strong>7)</strong> nigakilactone H and <strong>8)</strong> taxodione. On the other hand, <strong>C6</strong> showed a concentration-dependent cytotoxic effect on cancer cell lines MCF-7 (E<sub>max</sub> = 74.69 ± 6.19 % and IC<sub>50</sub> = 6.31 μg/mL), MDA-MB-231 (E<sub>max</sub> = 79.28 ± 12.12 % and IC<sub>50</sub> = 124.21 μg/mL), and SiHa (E<sub>max</sub> = 82.96 ± 6.02 % and IC<sub>50</sub> = 124.31 μg/mL). The <strong>C6</strong> did not show a cytotoxic effect against DU-145 and non-cancerous cells from the mammary glands MCF-10A. These results indicate cytotoxic specificity on cancer cell lines and support the hypothesis that terpenes identified in <em>E. mexicana</em> must be investigated and developed for non-clinical and clinical trials as potential anti-cancer drugs.</p></div>","PeriodicalId":12147,"journal":{"name":"Fitoterapia","volume":null,"pages":null},"PeriodicalIF":2.5000,"publicationDate":"2024-08-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Fitoterapia","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0367326X24003770","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"CHEMISTRY, MEDICINAL","Score":null,"Total":0}
引用次数: 0
Abstract
Epaltes mexicana is a plant widely used in traditional medicine and as a food in Mexico; however, its phytochemical and pharmacological studies are limited. This study aimed to identify the active secondary metabolites of Epaltes mexicana and determine its cytotoxic activity on cancer cell lines. Three organic extracts were obtained by maceration using n-hexane, dichloromethane, and methanol. The n-hexane extract was fractioned by simple column chromatography. Eight terpenes were annotated in collection 6 (C6) by LC-QTOF-MS using a gradient elution and Electrospray Ionization (ESI) in positive ion mode: 1) Gibberellin A15, 2) farfugin A, 3) dehydromyodesmone, 4) eremopetasitenin A1, 5) hydroxyisonobilin, 6) anhydrocinnzeylanine, 7) nigakilactone H and 8) taxodione. On the other hand, C6 showed a concentration-dependent cytotoxic effect on cancer cell lines MCF-7 (Emax = 74.69 ± 6.19 % and IC50 = 6.31 μg/mL), MDA-MB-231 (Emax = 79.28 ± 12.12 % and IC50 = 124.21 μg/mL), and SiHa (Emax = 82.96 ± 6.02 % and IC50 = 124.31 μg/mL). The C6 did not show a cytotoxic effect against DU-145 and non-cancerous cells from the mammary glands MCF-10A. These results indicate cytotoxic specificity on cancer cell lines and support the hypothesis that terpenes identified in E. mexicana must be investigated and developed for non-clinical and clinical trials as potential anti-cancer drugs.
期刊介绍:
Fitoterapia is a Journal dedicated to medicinal plants and to bioactive natural products of plant origin. It publishes original contributions in seven major areas:
1. Characterization of active ingredients of medicinal plants
2. Development of standardization method for bioactive plant extracts and natural products
3. Identification of bioactivity in plant extracts
4. Identification of targets and mechanism of activity of plant extracts
5. Production and genomic characterization of medicinal plants biomass
6. Chemistry and biochemistry of bioactive natural products of plant origin
7. Critical reviews of the historical, clinical and legal status of medicinal plants, and accounts on topical issues.