MiR-122, miR-133a, and miR-206 as potential biomarkers for post-mortem interval estimation.

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-10-01 Epub Date: 2024-08-29 DOI:10.1007/s13258-024-01559-x

Eun Ju Lee, Mingyoung Jeong, Haneul Lee, Min-A Je, Kwangmin Park, Dong Geon Lee, Xianglan Xuan, Sunghyun Kim, Sunyoung Park, Jungho Kim

{"title":"MiR-122, miR-133a, and miR-206 as potential biomarkers for post-mortem interval estimation.","authors":"Eun Ju Lee, Mingyoung Jeong, Haneul Lee, Min-A Je, Kwangmin Park, Dong Geon Lee, Xianglan Xuan, Sunghyun Kim, Sunyoung Park, Jungho Kim","doi":"10.1007/s13258-024-01559-x","DOIUrl":null,"url":null,"abstract":"Backgroud: Accurate estimation of post-mortem interval (PMI) is crucial in forensic investigations. MicroRNAs (miRNAs or miRs) are small non-coding RNAs that remain relatively stable within the cell nucleus despite post-mortem changes.Objective: We assessed three target genes (miR-122, miR-133a, and miR-206) for PMI estimation using 72 healthy adult male BALB/c mice exposed to two different temperatures (4 and 21℃) at nine different time points over 10 days.Methods: Initially, the stability of the two reference genes (RNU6B and 5 srRNA) was evaluated using gene stability analysis tools (Delta Ct, Best Keeper, and Genorm) to select the optimal reference gene. RNU6B was found to be the most stable endogenous control. Subsequently, the expression patterns of miR-122, miR-133a, and miR-206 were analyzed within a 10-day PMI period using the heart, skeletal muscle, liver, and brain tissues.Results: At 4℃, miR-122 levels significantly decreased on days 8 and 10 in all tissues, with only the liver showing significant changes at 21℃. MiR-133a decreased over time in the heart, muscles, and brain, showing a dramatic decrease on days 8 and 10 in the heart and muscles at both temperatures. Although miR-206 levels decreased over time in muscles and liver at 4 ℃, these increased in the brain at 21 ℃, with no expression changes in other organs.Conclusion: In summary, miR-122, miR-133a, and miR-206 are potential PMI markers in heart and skeletal muscle tissues.","PeriodicalId":1,"journal":{"name":"Accounts of Chemical Research","volume":null,"pages":null},"PeriodicalIF":16.4000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Accounts of Chemical Research","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s13258-024-01559-x","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/8/29 0:00:00","PubModel":"Epub","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}

引用次数: 0

Abstract

Backgroud: Accurate estimation of post-mortem interval (PMI) is crucial in forensic investigations. MicroRNAs (miRNAs or miRs) are small non-coding RNAs that remain relatively stable within the cell nucleus despite post-mortem changes.

Objective: We assessed three target genes (miR-122, miR-133a, and miR-206) for PMI estimation using 72 healthy adult male BALB/c mice exposed to two different temperatures (4 and 21℃) at nine different time points over 10 days.

Methods: Initially, the stability of the two reference genes (RNU6B and 5 srRNA) was evaluated using gene stability analysis tools (Delta Ct, Best Keeper, and Genorm) to select the optimal reference gene. RNU6B was found to be the most stable endogenous control. Subsequently, the expression patterns of miR-122, miR-133a, and miR-206 were analyzed within a 10-day PMI period using the heart, skeletal muscle, liver, and brain tissues.

Results: At 4℃, miR-122 levels significantly decreased on days 8 and 10 in all tissues, with only the liver showing significant changes at 21℃. MiR-133a decreased over time in the heart, muscles, and brain, showing a dramatic decrease on days 8 and 10 in the heart and muscles at both temperatures. Although miR-206 levels decreased over time in muscles and liver at 4 ℃, these increased in the brain at 21 ℃, with no expression changes in other organs.

Conclusion: In summary, miR-122, miR-133a, and miR-206 are potential PMI markers in heart and skeletal muscle tissues.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

MiR-122、miR-133a 和 miR-206 作为潜在的生物标记物，可用于尸检间隔估计。

背景：准确估计死后间隔（PMI）对法医调查至关重要。微RNA（miRNA或miRs）是一种小型非编码RNA，尽管死后会发生变化，但它们在细胞核内仍保持相对稳定：我们使用 72 只健康成年雄性 BALB/c 小鼠，在 10 天内的 9 个不同时间点暴露于两种不同温度（4℃和 21℃）下，评估了用于估计 PMI 的三个目标基因（miR-122、miR-133a 和 miR-206）：首先，使用基因稳定性分析工具（Delta Ct、Best Keeper 和 Genorm）评估了两个参考基因（RNU6B 和 5 srRNA）的稳定性，以选择最佳参考基因。结果发现 RNU6B 是最稳定的内源对照。随后，利用心脏、骨骼肌、肝脏和脑组织分析了 miR-122、miR-133a 和 miR-206 在 10 天 PMI 期间的表达模式：在 4℃ 时，所有组织的 miR-122 水平在第 8 天和第 10 天均显著下降，只有肝脏在 21℃ 时出现显著变化。心脏、肌肉和大脑中的 miR-133a 随着时间的推移而减少，在两种温度下，心脏和肌肉中的 miR-133a 在第 8 天和第 10 天都急剧减少。虽然4 ℃时肌肉和肝脏中的miR-206水平随时间下降，但21 ℃时大脑中的miR-206水平上升，其他器官中的表达没有变化：总之，miR-122、miR-133a 和 miR-206 是心脏和骨骼肌组织中潜在的 PMI 标志物。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.

期刊最新文献

Management of Cholesteatoma: Hearing Rehabilitation. Congenital Cholesteatoma. Evaluation of Cholesteatoma. Management of Cholesteatoma: Extension Beyond Middle Ear/Mastoid. Recidivism and Recurrence.