Serum chemistry, thyroxine concentration, and blood cell morphology of wild, young Blackchin Guitarfish Glaucostegus cemiculus sampled on the eastern Mediterranean coastline

IF 1.5 4区 农林科学 Q3 FISHERIES Journal of aquatic animal health Pub Date : 2024-09-01 DOI:10.1002/aah.10228
Nevo Schwartz, Danny Morick, Barak Azrieli, Aviad Scheinin, Dan Tchernov, Itamar Aroch
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在地中海东部海岸线采样的野生幼年黑琴鱼 Glaucostegus cemiculus 的血清化学成分、甲状腺素浓度和血细胞形态。
目的:黑琴鲉(Glaucostegus cemiculus)是地中海的特有物种,濒临灭绝,但却没有相关的常规实验室数据。我们的目标是测定充盈细胞体积(PCV)、综合血清化学分析指标和血清总甲状腺素(sTT4)浓度;比较用两种不同方法测定的血清白蛋白和血清球蛋白浓度;以及描述健康、自由活动的黑琴吉塔鱼的血细胞形态:方法:使用围网捕获野生黑琴吉塔鱼。方法:使用围网捕获野生黑琴鸡鱼,采集血样进行血清化学和血液学分析,并使用常规实验室方法进行测量。这些鱼被贴上标签后放生:根据总长度和体重估算,这项研究包括 43 条年龄小于 6 个月的黑琴鲉(17 条雄鱼和 26 条雌鱼)。PCV 中位数(n = 23)为 22%(最小值-最大值 [min-max] = 15-25%)。通过化学发光免疫测定法测得的 sTT4 中位数(n = 10)为 7.86 nmol/L(最小-最大值 = 7.52-9.57 nmol/L)。该研究包括全面的 25 种分析物血清化学分析(如血清铁、非结合铁和总铁结合能力)以及所有血细胞的形态学描述。血清电泳(SEP;n = 13)得出了一致的血清白蛋白迁移蛋白部分和四个球蛋白部分。血清电泳图证实了这些结果:结论:与溴甲酚绿分光光度法测量的结果相比,用 SEP 结合血清总蛋白浓度测量的黑琴鳄血清白蛋白迁移部分得出的白蛋白浓度要高得多。这种白蛋白迁移部分的真实身份仍有待确定。计算出的分析物 2.5-97.5% 百分位数间隔应被视为适用于年龄相近的黑琴鲉的参考间隔,但应用于成鱼时应谨慎。
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来源期刊
Journal of aquatic animal health
Journal of aquatic animal health 农林科学-兽医学
CiteScore
2.50
自引率
0.00%
发文量
25
审稿时长
>24 weeks
期刊介绍: The Journal of Aquatic Animal Health serves the international community of scientists and culturists concerned with the health of aquatic organisms. It carries research papers on the causes, effects, treatments, and prevention of diseases of marine and freshwater organisms, particularly fish and shellfish. In addition, it contains papers that describe biochemical and physiological investigations into fish health that relate to assessing the impacts of both environmental and pathogenic features.
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Issue Information Serum chemistry, thyroxine concentration, and blood cell morphology of wild, young Blackchin Guitarfish Glaucostegus cemiculus sampled on the eastern Mediterranean coastline Adipose tissue torsion with localized lymphangiectasia in a Rio Cauca caecilian Typhlonectes natans Oxidative response to cadmium and lead accumulations in the tissues of blue swimming crabs Portunus pelagicus from the Trang Province coastline, Southern Thailand A perspective: Molecular detections of new agents in finfish—Interpreting biological significance for fish health management: Response to comment
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