Diffusion- and Perfusion-Weighted Imaging to Detect Neurological Deficits in Acute Focal Cerebral Ischemia in Rabbits.

IF 2.5 4区医学 Q3 NEUROSCIENCES Journal of integrative neuroscience Pub Date : 2024-08-21 DOI:10.31083/j.jin2308156

Yu Zhang, Xuefei Deng, Jiangdong Chu, Qian Zhang, Xiangwei Luo, Xingxing Wang

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Abstract

Purpose: To investigate the relationship of diffusion-weighted imaging (DWI) and perfusion-weighted imaging (PWI) parameters with dysfunction in acute focal cerebral ischemia (ACI) rabbits.

Methods: The model of ACI in the middle cerebral artery was made using 30 adult male New Zealand rabbits. The dysfunction severities of the ACI rabbits were assessed using Purdy's score. A paired-sample rank sum test was adopted to compare the abnormal signal zone (ASZ) volumes from T₂ weighted imaging (T₂WI), dynamic susceptibility contrast-enhanced (DSC) imaging, and DWI with a relative cerebral blood flow (rCBF) map; correlations were analyzed between the volume of each ASZ and Purdy's score by Spearman's rank correlation coefficient. The degree of necrotic and apoptotic cells was evaluated in the ASZ from DWI and DSC PWI-DWI mismatch (PDM) zone. Correlations were analyzed between the index of cellular damage and Purdy's score, the volume of ASZs by Spearman's rank correlation coefficient.

Results: The ASZ volumes from DSC-PWI and the rCBF maps were larger than those from DWI (p < 0.001 and p < 0.001, respectively); those from the rCBF map (Z = 0.959, p < 0.001) and DSC-PWI (Z = 0.970, p < 0.001) were positively correlated with DWI; a positive correlation was found between Purdy's score and the ASZ volumes from DSC-PWI (Z = 0.889, p < 0.001), DWI (Z = 0.921, p < 0.001), and rCBF (Z = 0.891, p < 0.001). A significant difference was observed between the ASZ from DWI and the PDM zone in terms of the degree of necrotic (p < 0.001) and apoptotic cells (p < 0.001). The degree of cellular damage in the ASZ of DWI and PDM zone had no relationship with Purdy's score and the volumes of ASZs.

Conclusion: The ASZ volumes from DSC-PWI, rCBF, and particularly DWI reflected the level of dysfunction in rabbits with ACI.

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用弥散加权成像和灌注加权成像检测兔子急性局灶性脑缺血的神经功能缺损。

目的：探讨弥散加权成像（DWI）和灌注加权成像（PWI）参数与急性局灶性脑缺血（ACI）兔功能障碍的关系：方法：使用 30 只成年雄性新西兰兔制作大脑中动脉 ACI 模型。方法：使用 30 只成年雄性新西兰兔制作大脑中动脉 ACI 模型，并使用 Purdy 评分法评估 ACI 兔的功能障碍严重程度。采用配对样本秩和检验比较 T2 加权成像（T2WI）、动态感性对比增强成像（DSC）和 DWI 的异常信号区（ASZ）体积与相对脑血流（rCBF）图；采用 Spearman 秩相关系数分析各 ASZ 体积与 Purdy 评分之间的相关性。根据 DWI 和 DSC PWI-DWI 不匹配（PDM）区评估 ASZ 中坏死和凋亡细胞的程度。通过斯皮尔曼秩相关系数分析了细胞损伤指数与Purdy评分、ASZ体积之间的相关性：结果：DSC-PWI和rCBF图的ASZ体积大于DWI的ASZ体积（分别为p < 0.001和p < 0.001）；rCBF图的ASZ体积（Z = 0.959，p < 0.001）和DSC-PWI的ASZ体积（Z = 0.970，p < 0.001）与 DWI 呈正相关；Purdy 评分与来自 DSC-PWI（Z = 0.889，p < 0.001）、DWI（Z = 0.921，p < 0.001）和 rCBF（Z = 0.891，p < 0.001）的 ASZ 体积呈正相关。从 DWI 观察到的 ASZ 和 PDM 区在坏死细胞（p < 0.001）和凋亡细胞（p < 0.001）的程度上存在明显差异。DWI和PDM区ASZ的细胞损伤程度与Purdy评分和ASZ体积没有关系：结论：DSC-PWI、rCBF，尤其是 DWI 显示的 ASZ 体积反映了 ACI 兔的功能障碍程度。

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来源期刊

Journal of integrative neuroscience 医学-神经科学

CiteScore

2.80

自引率

5.60%

发文量

173

审稿时长

2 months

期刊介绍： JIN is an international peer-reviewed, open access journal. JIN publishes leading-edge research at the interface of theoretical and experimental neuroscience, focusing across hierarchical levels of brain organization to better understand how diverse functions are integrated. We encourage submissions from scientists of all specialties that relate to brain functioning.