Physical radiation induced the yield of triterpenoids in hypha of Inonotus obliquus to increase

IF 1.7 4区生物学 Q4 BIOCHEMICAL RESEARCH METHODS Journal of microbiological methods Pub Date : 2024-08-30 DOI:10.1016/j.mimet.2024.107025

Yan Wang , Xin Liu , Lirui Sun , Boxin Dou , Jiaying Xin , Na Zhang , Lanwei Zhang

引用次数: 0

Abstract

HSD-IO01, a new pure strain of I. obliquus, was isolated and purified from the sclerotium of I. obliquus of Daxing'an Mountains. Physical radiation-assisted liquid fermentation technology was explored to increase the triterpenoids yield of HSD-IO01. In the 100 mL optimized liquid fermentation system, the hypha dry weight of HSD-IO01 was 1.7734 g, and the triterpenoids yield was 43.43 mg. Yields of triterpenoids increased after induction with ultrasound, microwave, or UV light, respectively. Among them, ultrasonic treatment had the most remarkable induction effect. The yield of triterpenoids would be increased to 68.35 mg (57.38 %) when the HSD-IO01 was treated by 100 W ultrasonic for 45 min. Establishing ultrasonic-assisted liquid fermentation technology could further promote the detailed development and comprehensive utilization of I. obliquus resources.

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物理辐射诱导斜纹夜蛾菌丝中三萜类化合物的产量增加。

HSD-IO01是从大兴安岭斜纹夜蛾硬核菌中分离纯化的一株新的斜纹夜蛾纯菌株。为提高 HSD-IO01 的三萜类化合物产量，探索了物理辐射辅助液体发酵技术。在 100 mL 优化液体发酵体系中，HSD-IO01 的菌丝干重为 1.7734 g，三萜类化合物产量为 43.43 mg。经超声波、微波或紫外线诱导后，三萜类化合物的产量分别增加。其中，超声波处理的诱导效果最为显著。用 100 W 超声波处理 HSD-IO01 45 分钟后，三萜类化合物的产量增加到 68.35 毫克（57.38%）。超声波辅助液体发酵技术的建立可进一步促进藻类资源的详细开发和综合利用。

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来源期刊

Journal of microbiological methods 生物-生化研究方法

CiteScore

4.30

自引率

4.50%

发文量

151

审稿时长

29 days

期刊介绍： The Journal of Microbiological Methods publishes scholarly and original articles, notes and review articles. These articles must include novel and/or state-of-the-art methods, or significant improvements to existing methods. Novel and innovative applications of current methods that are validated and useful will also be published. JMM strives for scholarship, innovation and excellence. This demands scientific rigour, the best available methods and technologies, correctly replicated experiments/tests, the inclusion of proper controls, calibrations, and the correct statistical analysis. The presentation of the data must support the interpretation of the method/approach. All aspects of microbiology are covered, except virology. These include agricultural microbiology, applied and environmental microbiology, bioassays, bioinformatics, biotechnology, biochemical microbiology, clinical microbiology, diagnostics, food monitoring and quality control microbiology, microbial genetics and genomics, geomicrobiology, microbiome methods regardless of habitat, high through-put sequencing methods and analysis, microbial pathogenesis and host responses, metabolomics, metagenomics, metaproteomics, microbial ecology and diversity, microbial physiology, microbial ultra-structure, microscopic and imaging methods, molecular microbiology, mycology, novel mathematical microbiology and modelling, parasitology, plant-microbe interactions, protein markers/profiles, proteomics, pyrosequencing, public health microbiology, radioisotopes applied to microbiology, robotics applied to microbiological methods,rumen microbiology, microbiological methods for space missions and extreme environments, sampling methods and samplers, soil and sediment microbiology, transcriptomics, veterinary microbiology, sero-diagnostics and typing/identification.