Ulva lactuca polysaccharides combined with fecal microbiota transplantation ameliorated dextran sodium sulfate-induced colitis in C57BL/6J mice

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY Journal of the Science of Food and Agriculture Pub Date : 2024-08-30 DOI:10.1002/jsfa.13839

Zhengqi Liu, Menghui Wang, Yuanyuan Hu, Jinjin Li, Wei Gong, Xiaoming Guo, Shuang Song, Beiwei Zhu

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Abstract

BACKGROUND

Fecal microbiota transplantation (FMT) of healthy donors improves ulcerative colitis (UC) patients by restoring the balance of the gut microbiota. However, donors vary in microbial diversity and composition, often resulting in weak or even ineffective FMT. Improving the efficacy of FMT through combination treatment has become a promising strategy. Ulva lactuca polysaccharides (ULP) have been found to benefit host health by regulating gut microbiota. The effect of the combination of ULP and FMT in ameliorating UC has not yet been evaluated.

RESULTS

The present study found that supplementation with ULP combined with FMT showed better effects in ameliorating UC than supplementation with FMT alone. Results suggested that FMT or ULP combined with FMT alleviated the symptoms of UC in mice, as evidenced by prevention of body weight loss, improvement of disease activity index and protection of the intestinal mucus. Notably, ULP in combination with FMT was more effective than FMT in reducing levels of cytokines and related inflammatory enzymes. In addition, ULP combined with FMT effectively restored the dysbiosis induced by dextran sulfate sodium (DSS) and further enriched probiotics (such as Bifidobacterium). The production of short-chain fatty acids, especially acetic acid, was also significantly enriched by ULP combined with FMT.

CONCLUSION

Supplementation of ULP combined with FMT could significantly ameliorate DSS-induced colitis in mice by inhibiting inflammation and restoring dysbiosis of gut microbiota. These results suggested that ULP combined with FMT has potential application in ameliorating UC. © 2024 Society of Chemical Industry.

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乳莼多糖与粪便微生物群移植相结合可改善右旋糖酐硫酸钠诱导的 C57BL/6J 小鼠结肠炎。

背景：健康供体的粪便微生物群移植（FMT）可恢复肠道微生物群的平衡，从而改善溃疡性结肠炎（UC）患者的病情。然而，供体的微生物多样性和组成各不相同，往往导致 FMT 效果不佳甚至无效。通过联合治疗提高 FMT 的疗效已成为一种前景广阔的策略。研究发现，莼菜多糖（ULP）可通过调节肠道微生物群来有益于宿主健康。目前尚未评估 ULP 和 FMT 联合治疗对改善 UC 的效果：结果：本研究发现，补充 ULP 和 FMT 对改善 UC 的效果优于单独补充 FMT。结果表明，FMT 或 ULP 联合 FMT 可减轻小鼠的 UC 症状，具体表现为防止体重下降、改善疾病活动指数和保护肠粘液。值得注意的是，在降低细胞因子和相关炎症酶水平方面，ULP 联合 FMT 比 FMT 更有效。此外，ULP 联合 FMT 还能有效恢复右旋糖酐硫酸钠（DSS）引起的菌群失调，并进一步丰富益生菌（如双歧杆菌）。ULP联合FMT还显著提高了短链脂肪酸，尤其是乙酸的产量：结论：通过抑制炎症和恢复肠道微生物群的菌群失调，补充 ULP 和 FMT 可明显改善 DSS 诱导的小鼠结肠炎。这些结果表明，ULP 联合 FMT 有可能用于改善 UC。© 2024 化学工业协会。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

期刊介绍： The Journal of the Science of Food and Agriculture publishes peer-reviewed original research, reviews, mini-reviews, perspectives and spotlights in these areas, with particular emphasis on interdisciplinary studies at the agriculture/ food interface. Published for SCI by John Wiley & Sons Ltd. SCI (Society of Chemical Industry) is a unique international forum where science meets business on independent, impartial ground. Anyone can join and current Members include consumers, business people, environmentalists, industrialists, farmers, and researchers. The Society offers a chance to share information between sectors as diverse as food and agriculture, pharmaceuticals, biotechnology, materials, chemicals, environmental science and safety. As well as organising educational events, SCI awards a number of prestigious honours and scholarships each year, publishes peer-reviewed journals, and provides Members with news from their sectors in the respected magazine, Chemistry & Industry . Originally established in London in 1881 and in New York in 1894, SCI is a registered charity with Members in over 70 countries.