Insights into the Evolutionary Dynamics: Characterization of Disintegrin and Metalloproteinase Proteins in the Venom Gland Transcriptome of the Hemiscorpius lepturus Scorpion.

IF 1 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Protein and Peptide Letters Pub Date : 2024-08-30 DOI:10.2174/0109298665321842240819073453

Abbas Rami, Benjamin Damizadeh, Mahdi Behdani, Fatemeh Kazemi-Lomedasht

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Abstract

Background: The Disintegrin and Metalloproteinase (ADAM) family, also known as the metalloproteinase/disintegrin/cysteine-rich (MDC) proteins, includes both secreted and transmembrane molecules involved in critical biological processes, such as cell migration, adhesion, and signaling. This study aimed to investigate the evolutionary relationships and structural characteristics of disintegrin and metalloproteinase proteins identified in the venom gland transcriptome of the scorpion Hemiscorpius lepturus.

Methods: Using bioinformatics tools, we analyzed the open reading frame, conserved motifs, and primary, secondary, and tertiary structures of these proteins. Five proteins, named HLDis- Met1, HLDisMet2, HLDisMet3, HLDisMet4, and HLDisMet5, were identified. Their predicted 3- D structures were within normal ranges (Z-score between -4 to -9).

Results: Phylogenetic analysis revealed that HLDisMet1 shares similarities with proteins from various spider species (Nephila pilipes, Argiope bruennichi, Araneus ventricosus, and Trichonephila inaurata madagascariensis), HLDisMet2 with the scorpion Centruroides sculpturatus, HLDis- Met4 with the scorpion Tityus serrulatus, and HLDisMet5 with several snake species (Python bivittatus, Vipera anatolica senliki, Protobothrops mucrosquamatus, and Naja naja).

Conclusion: These findings highlight the significant similarities between HLDisMet proteins and those found in other venomous species, suggesting a complex and diverse evolutionary pathway for venom components. The cross-species conservation observed may indicate a convergent evolutionary strategy, where different species independently develop similar venom components to adapt to similar ecological niches or prey types. This study highlights the evolutionary significance of venom diversification and its potential applications in understanding venom biology across different species.

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洞察进化动态：Hemiscorpius lepturus Scorpion 毒腺转录组中分解蛋白和金属蛋白酶蛋白的特征。

背景：解体蛋白酶和金属蛋白酶（ADAM）家族又称金属蛋白酶/解体蛋白酶/富半胱氨酸（MDC）蛋白，包括分泌型和跨膜型分子，参与细胞迁移、粘附和信号传导等关键生物过程。本研究旨在研究在蝎子 Hemiscorpius lepturus 毒腺转录组中发现的崩解素和金属蛋白酶蛋白的进化关系和结构特征：我们使用生物信息学工具分析了这些蛋白的开放阅读框、保守基序以及一级、二级和三级结构。结果发现了五种蛋白质，分别命名为HLDis- Met1、HLDisMet2、HLDisMet3、HLDisMet4和HLDisMet5。它们预测的 3- D 结构在正常范围内（Z-score 在-4 到-9 之间）：系统进化分析表明，HLDisMet1与多种蜘蛛（Nephila pilipes、Argiope bruennichi、Araneus ventricosus和Trichonephila inaurata madagascariensis）的蛋白质具有相似性、HLDisMet2 与蝎子 Centruroides sculpturatus 相同，HLDis- Met4 与蝎子 Tityus serrulatus 相同，HLDisMet5 与几种蛇类（Python bivittatus、Vipera anatolica senliki、Protobothrops mucrosquamatus 和 Naja naja）相同。结论这些发现凸显了 HLDisMet 蛋白与其他毒液物种中的 HLDisMet 蛋白之间的显著相似性，表明毒液成分的进化途径复杂多样。所观察到的跨物种保护可能表明了一种趋同的进化策略，即不同物种独立发展出类似的毒液成分，以适应类似的生态位或猎物类型。这项研究强调了毒液多样化的进化意义及其在理解不同物种毒液生物学方面的潜在应用。

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来源期刊

Protein and Peptide Letters 生物-生化与分子生物学

CiteScore

2.90

自引率

0.00%

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审稿时长

2 months

期刊介绍： Protein & Peptide Letters publishes letters, original research papers, mini-reviews and guest edited issues in all important aspects of protein and peptide research, including structural studies, advances in recombinant expression, function, synthesis, enzymology, immunology, molecular modeling, and drug design. Manuscripts must have a significant element of novelty, timeliness and urgency that merit rapid publication. Reports of crystallization and preliminary structure determination of biologically important proteins are considered only if they include significant new approaches or deal with proteins of immediate importance, and preliminary structure determinations of biologically important proteins. Purely theoretical/review papers should provide new insight into the principles of protein/peptide structure and function. Manuscripts describing computational work should include some experimental data to provide confirmation of the results of calculations. Protein & Peptide Letters focuses on: Structure Studies Advances in Recombinant Expression Drug Design Chemical Synthesis Function Pharmacology Enzymology Conformational Analysis Immunology Biotechnology Protein Engineering Protein Folding Sequencing Molecular Recognition Purification and Analysis