Exploration of Genetic Variation and Population Structure in Bergenia ciliata for its Conservation Implications.

IF 2.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemical Genetics Pub Date : 2024-09-02 DOI:10.1007/s10528-024-10908-0
Harish Chandra Singh, Vandana Tiwari, Baleshwar Meena, Avinash Tiwari, Tikam Singh Rana
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Abstract

Bergenia ciliata (Haw.) Sternb. is a perennial medicinal herb distributed in Indian Himalayan Region (IHR). A total of eight populations of B. ciliata were collected from diverse locales of IHR, and 17 EST-SSR markers were used in this study. The present study revealed moderate genetic diversity at the locus level with the mean number of alleles (Na = 7.823), mean number effective of alleles (Ne = 3.375), mean expected heterozygosity (He = 0.570), and mean Shannon's diversity index (I = 1.264). The MSR (He = 0.543, I = 1.067) and DRJ populations (He = 0.309, I = 0.519) revealed the highest and lowest genetic diversity at the population level, respectively. AMOVA analysis showed that 81.76% of genetic variation was within populations, 10.55% was among populations, and 7.69% was among the regions. In addition, a moderate to high level of differentiation was found among the populations (FST = 0.182), which could be indicative of low to moderate gene flow (Nm = 0.669) in the B. ciliata populations. UPGMA and PCoA analysis revealed that eight populations could be differentiated into two groups, while the structure analysis of the 96 individuals differentiated into three groups. The Mantel test showed a positive relationship between genetic and geographical distance. The findings of this study will provide the development of conservation and germplasm management strategies for this valuable medicinal species.

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探索纤毛虫的遗传变异和种群结构对保护工作的影响。
Bergenia ciliata (Haw.) Sternb.是一种多年生药用植物,分布于印度喜马拉雅地区(IHR)。本研究从印度喜马拉雅地区的不同地方共收集了 8 个纤毛虫种群,并使用了 17 个 EST-SSR 标记。本研究显示,等位基因的平均数量(Na = 7.823)、等位基因的平均有效数量(Ne = 3.375)、平均预期杂合度(He = 0.570)和平均香农多样性指数(I = 1.264)在位点水平上具有适度的遗传多样性。MSR 种群(He = 0.543,I = 1.067)和 DRJ 种群(He = 0.309,I = 0.519)在种群水平上的遗传多样性分别最高和最低。AMOVA分析表明,81.76%的遗传变异发生在种群内部,10.55%发生在种群之间,7.69%发生在区域之间。此外,种群间的分化程度为中高水平(FST = 0.182),这可能表明纤毛虫种群中存在中低水平的基因流(Nm = 0.669)。UPGMA 和 PCoA 分析表明,8 个种群可分为两组,而 96 个个体的结构分析则可分为三组。Mantel 检验表明,遗传距离和地理距离之间存在正相关关系。这项研究的结果将为这一珍贵药用物种的保护和种质管理策略的制定提供参考。
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来源期刊
Biochemical Genetics
Biochemical Genetics 生物-生化与分子生物学
CiteScore
3.90
自引率
0.00%
发文量
133
审稿时长
4.8 months
期刊介绍: Biochemical Genetics welcomes original manuscripts that address and test clear scientific hypotheses, are directed to a broad scientific audience, and clearly contribute to the advancement of the field through the use of sound sampling or experimental design, reliable analytical methodologies and robust statistical analyses. Although studies focusing on particular regions and target organisms are welcome, it is not the journal’s goal to publish essentially descriptive studies that provide results with narrow applicability, or are based on very small samples or pseudoreplication. Rather, Biochemical Genetics welcomes review articles that go beyond summarizing previous publications and create added value through the systematic analysis and critique of the current state of knowledge or by conducting meta-analyses. Methodological articles are also within the scope of Biological Genetics, particularly when new laboratory techniques or computational approaches are fully described and thoroughly compared with the existing benchmark methods. Biochemical Genetics welcomes articles on the following topics: Genomics; Proteomics; Population genetics; Phylogenetics; Metagenomics; Microbial genetics; Genetics and evolution of wild and cultivated plants; Animal genetics and evolution; Human genetics and evolution; Genetic disorders; Genetic markers of diseases; Gene technology and therapy; Experimental and analytical methods; Statistical and computational methods.
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