Metagenomics Analysis Reveals Unique Gut Microbiota Signature of Slow-Transit Constipation.

IF 3 3区医学 Q2 GASTROENTEROLOGY & HEPATOLOGY Clinical and Translational Gastroenterology Pub Date : 2024-10-01 DOI:10.14309/ctg.0000000000000766

Kyungsun Han, Braden Kuo, Hamed Khalili, Kyle Staller

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Abstract

Introduction: Altered gut microbiota may play a role in slow-transit constipation (STC). We conducted a study of gut microbiota composition and functionality in STC using metagenomic analyses.

Methods: We assembled a clinical cohort of 24 patients with STC physiology age- and sex-matched to 24 controls. We performed shotgun metagenomic sequencing followed by prediction of metabolite composition from functional profiles.

Results: In a middle-aged (mean 55.3 years), predominantly female cohort, there were no significant differences in α-diversity indices, but permutational multivariate analysis of variance analysis showed significant between-group differences (R 2 = 0.050, P < 0.001) between STC patients and controls. Gordonibacter pamelaeae , Bifidobacterium longum , Firmicutes bacterium co-abundance gene group 94, and Anaerotruncus colihominis were more abundant in STC, whereas Coprococcus comes and Roseburia intestinalis were more abundant in controls. Gut-derived metabolites varying in STC relative to controls were related to bile acid and cholesterol metabolism.

Discussion: We found a unique metagenomic and metabolomic signature of STC.

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元基因组学分析揭示了缓慢型便秘的独特肠道微生物群特征。

简介：肠道微生物群的改变可能是导致慢传输性便秘（STC）的原因之一。我们利用元基因组分析法对慢行性便秘患者的肠道微生物群组成和功能进行了研究：方法：我们组建了一个临床队列，其中包括 24 名与 24 名对照组年龄和性别相匹配的 STC 生理病患者。我们进行了霰弹枪元基因组测序，然后根据功能图谱预测代谢物组成：结果：在以女性为主的中年（平均 55.3 岁）组群中，α 多样性指数无显著差异，但 permutational 多变量方差分析显示组间差异显著（R2=0.050，p 讨论：我们发现了 STC 独特的元基因组和代谢组特征。

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来源期刊

Clinical and Translational Gastroenterology GASTROENTEROLOGY & HEPATOLOGY-

CiteScore

7.00

自引率

0.00%

发文量

114

审稿时长

16 weeks

期刊介绍： Clinical and Translational Gastroenterology (CTG), published on behalf of the American College of Gastroenterology (ACG), is a peer-reviewed open access online journal dedicated to innovative clinical work in the field of gastroenterology and hepatology. CTG hopes to fulfill an unmet need for clinicians and scientists by welcoming novel cohort studies, early-phase clinical trials, qualitative and quantitative epidemiologic research, hypothesis-generating research, studies of novel mechanisms and methodologies including public health interventions, and integration of approaches across organs and disciplines. CTG also welcomes hypothesis-generating small studies, methods papers, and translational research with clear applications to human physiology or disease. Colon and small bowel Endoscopy and novel diagnostics Esophagus Functional GI disorders Immunology of the GI tract Microbiology of the GI tract Inflammatory bowel disease Pancreas and biliary tract Liver Pathology Pediatrics Preventative medicine Nutrition/obesity Stomach.