A simplified metabolomic analysis of dried blood spots in breast cancer patients.

IF 1.3 4区 医学 Q4 MEDICINE, RESEARCH & EXPERIMENTAL Scandinavian Journal of Clinical & Laboratory Investigation Pub Date : 2024-09-01 Epub Date: 2024-09-03 DOI:10.1080/00365513.2024.2392241
Georgia Thodi, Aikaterini Triantopoulou, Aikaterini Iliou, Elina Molou, Yannis Dotsikas, Yannis L Loukas
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Abstract

Breast cancer (BC) is among the most commonly diagnosed cancers. Besides mammography, breast ultrasonography and the routinely monitored protein markers, the variations of small molecular metabolites in blood may be of great diagnostic value. This study aimed to quantify specific metabolite markers with potential application in BC detection. The study enrolled 50 participants, 25 BC patients and 25 healthy controls (CTRL). Dried blood spots (DBS) were utilized as biological media and were quantified via a simplified liquid chromatography tandem mass spectrometry (LC-MS/MS) method, used in expanded newborn screening. The targeted metabolomic analysis included 12 amino acids and 32 acylcarnitines. Statistical analysis revealed a significant variation of metabolic profiles between BC patients and CTRL. Among the 44 metabolites, 18 acylcarnitines and 10 amino acids remained significant after Bonferroni correction, showing increase or decrease and enabled classification of BC patients and CTRL. The well-established LC-MS/MS protocol could provide results within few minutes. Therefore, the combination of an easy-to-handle material-DBS and LC-MS/MS protocol could facilitate BC screening/diagnosis and in the next step applied to other cancer patients, as well.

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乳腺癌患者干血斑的简化代谢组学分析。
乳腺癌(BC)是最常见的癌症之一。除了乳腺X光检查、乳腺超声波检查和常规监测的蛋白质标志物外,血液中小分子代谢物的变化可能具有重要的诊断价值。本研究旨在量化可能应用于乳腺癌检测的特定代谢物标记物。该研究共招募了 50 名参与者,其中包括 25 名 BC 患者和 25 名健康对照者(CTRL)。研究人员使用干血斑(DBS)作为生物媒介,并通过用于新生儿扩大筛查的简化液相色谱串联质谱法(LC-MS/MS)进行定量分析。目标代谢组分析包括 12 种氨基酸和 32 种酰基肉碱。统计分析显示,BC 患者和 CTRL 患者的代谢谱存在明显差异。在 44 种代谢物中,18 种酰基肉碱和 10 种氨基酸经 Bonferroni 校正后仍具有显著性,显示出增加或减少的情况,并可对 BC 患者和 CTRL 进行分类。成熟的 LC-MS/MS 程序可在几分钟内得出结果。因此,将易于处理的材料--DBS 和 LC-MS/MS 方案结合起来,可以促进对 BC 的筛查/诊断,并在下一步应用于其他癌症患者。
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来源期刊
CiteScore
3.50
自引率
4.80%
发文量
85
审稿时长
4-8 weeks
期刊介绍: The Scandinavian Journal of Clinical and Laboratory Investigation is an international scientific journal covering clinically oriented biochemical and physiological research. Since the launch of the journal in 1949, it has been a forum for international laboratory medicine, closely related to, and edited by, The Scandinavian Society for Clinical Chemistry. The journal contains peer-reviewed articles, editorials, invited reviews, and short technical notes, as well as several supplements each year. Supplements consist of monographs, and symposium and congress reports covering subjects within clinical chemistry and clinical physiology.
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