Aerosolization ocular surface microorganisms accumulation effect during non-contact tonometer measurements.

IF 1.7 4区医学 Q3 OPHTHALMOLOGY BMC Ophthalmology Pub Date : 2024-09-03 DOI:10.1186/s12886-024-03664-7

Xinyi Shen, Yi Xu, Yuee Ye, Shuo Huai, Peiyu Wu, Jinzhi Huang, Weihe Zhou, Chunchun Li, Yanyan Chen

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Abstract

Purpose: This study aimed to verify that aerosolization ocular surface microorganisms (AOSMs) accumulated during non-contact tonometry (NCT) measurements.

Methods: A total of 508 participants (740 eyes) were enrolled in the study. In Experiment 1, before NCT was performed on each eye, the air was disinfected, and environment air control samples were collected via Air ideal^® 3P (Bio Merieux). During NCT measurements, microbial aerosol samples were collected once from each eye. In Experiment 2, we collected initial blank control samples and then repeated Experiment 1. Finally, in Experiment 3, after the background microbial aerosol investigation, we cumulatively sampled AOSMs from each 10 participants then culture once, without any interventions to interrupt the accumulation. The collected samples were incubated and identified using matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF-MS).

Results: Pathogenic Aerococcus viridans and other microorganisms from human eyes can spread and accumulate in the air during NCT measurements. The species and quantity of AOSMs produced by NCT measurements can demonstrate an accumulation effect.

Conclusion: AOSMs generated during NCT measurements are highly likely to spread and accumulate in the air, thereby may increase the risk of exposure to and transmission of bio-aerosols.

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非接触式眼压计测量过程中眼球表面微生物积累的气溶胶效应。

目的：本研究旨在验证在非接触式眼压计（NCT）测量过程中眼球表面微生物（AOSMs）的积累情况：方法：共有 508 名参与者（740 只眼睛）参加了研究。在实验 1 中，在对每只眼睛进行 NCT 测量前，对空气进行消毒，并通过 Air ideal® 3P (Bio Merieux) 收集环境空气控制样本。在 NCT 测量期间，从每只眼睛采集一次微生物气溶胶样本。在实验 2 中，我们先收集空白对照样本，然后重复实验 1。最后，在实验 3 中，在背景微生物气溶胶调查之后，我们从每 10 位参与者的 AOSMs 中累积采样，然后培养一次，不采取任何干预措施中断累积。对收集的样本进行培养，并使用基质辅助激光解吸/电离飞行时间质谱法（MALDI-TOF-MS）进行鉴定：结果：在 NCT 测量过程中，来自人眼的致病性病毒性厌氧球菌和其他微生物会在空气中扩散和积累。结论：NCT 测量过程中产生的 AOSMs 的种类和数量可以证明积累效应：结论：NCT 测量过程中产生的 AOSMs 极有可能在空气中扩散和积累，从而增加接触和传播生物气溶胶的风险。

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来源期刊

BMC Ophthalmology OPHTHALMOLOGY-

CiteScore

3.40

自引率

5.00%

发文量

441

审稿时长

6-12 weeks

期刊介绍： BMC Ophthalmology is an open access, peer-reviewed journal that considers articles on all aspects of the prevention, diagnosis and management of eye disorders, as well as related molecular genetics, pathophysiology, and epidemiology.