HPTLC-guided flash chromatographic isolation and spectroscopic identification of bioactive compounds from olive flowers

IF 3.8 2区化学 Q1 BIOCHEMICAL RESEARCH METHODS Journal of Chromatography A Pub Date : 2024-08-31 DOI:10.1016/j.chroma.2024.465310

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Abstract

The goal of preparative chromatography is to isolate suitable amounts of compound(s) at the required purity in the most cost-effective way. This study analyses the power of High-performance thin-layer chromatography (HPTLC) guided preparative flash chromatography to separate and isolate bioactive compounds from an olive flower extract for their further characterisation via spectroscopy. The structure and purity of isolated bioactive compounds were assessed using Fourier-transform infrared (FTIR) and nuclear magnetic resonance (NMR) spectroscopy. Flash chromatography of the olive flower extract successfully isolated pure oleanolic and maslinic acids. Moreover, the flash chromatography of the extract allowed isolation and phytochemical analysis of the most lipophilic fraction of the extract, which was found to contain n-eicosane and n-(Z)-eicos-5-ene, that has not been isolated previously with preparative TLC.

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HPTLC 引导的闪速色谱分离和光谱鉴定橄榄花中的生物活性化合物

制备色谱法的目标是以最具成本效益的方式分离出适量、纯度符合要求的化合物。本研究分析了高效薄层色谱（HPTLC）引导的制备闪蒸色谱法从橄榄花提取物中分离和分离生物活性化合物的能力，以便通过光谱分析进一步确定其特征。利用傅立叶变换红外光谱（FTIR）和核磁共振光谱（NMR）对分离出的生物活性化合物的结构和纯度进行了评估。橄榄花提取物的闪色谱法成功分离出了纯净的齐墩果酸和马斯林酸。此外，通过对提取物进行闪蒸色谱分析，还分离出了提取物中亲脂性最强的馏分并对其进行了植物化学分析，发现其中含有正二十烷和正-(Z)-二十烷-5-烯，而这两种物质以前从未通过制备型 TLC 分离出来。

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来源期刊

Journal of Chromatography A 化学-分析化学

CiteScore

7.90

自引率

14.60%

发文量

742

审稿时长

45 days

期刊介绍： The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.