Knockdown of PRDX2 Inhibits the Proliferation, Growth, Migration, Invasion, and MMP9 Activity of Ewing's Sarcoma Cells Cultured In Vitro

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2024-09-05 DOI:10.1002/cnr2.2122

Ruifeng Xue, Zhengfu Fan, Yunhe An

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Abstract

Background

Ewing’s sarcoma (ES) is the second most common malignant primary bone tumor in children and adolescents. Peroxiredoxin 2 (PRDX2) is an antioxidant enzyme.

Aims

Here, we investigated the role and mechanism of PRDX2 in the development of ES.

Methods and results

PRDX2 expression was knocked down in A673 and RDES cells by specific siRNA interference (si-PRDX2). Knockdown of PRDX2 strongly inhibited the proliferation, growth, migration, invasion, and MMP9 activity and induces apoptosis of A673 and RDES cells. si-PRDX2 significantly inhibited the phosphorylation of Akt and the expression of cyclin D1. The transcription factor that might regulate PRDX2 transcription was predicted with the JASPAR and UCSC databases, and analyzed using dual-luciferase and Chromatin co-immunoprecipitation experiments. SNAI1 could activate the transcription of PRDX2 by binding to predicted promoter binding site.

Conclusion

PRDX2 may be a potential therapeutic target for ES.

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敲除 PRDX2 可抑制体外培养的尤文氏肉瘤细胞的增殖、生长、迁移、侵袭和 MMP9 活性。

背景：尤文氏肉瘤（ES）是儿童和青少年中第二常见的恶性原发性骨肿瘤。目的：在此，我们研究了PRDX2在ES发病中的作用和机制：通过特异性 siRNA 干扰（si-PRDX2）敲除 A673 和 RDES 细胞中 PRDX2 的表达。si-PRDX2 能显著抑制 Akt 的磷酸化和细胞周期蛋白 D1 的表达。利用JASPAR和UCSC数据库预测了可能调控PRDX2转录的转录因子，并利用双荧光素酶和染色质共免疫沉淀实验进行了分析。SNAI1可通过与预测的启动子结合位点结合来激活PRDX2的转录：结论：PRDX2可能是ES的潜在治疗靶点。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.