Development and validation of a simple chromatographic method to screen oral fluid samples for drugs in DUID investigations.

IF 2.3 3区医学 Q3 CHEMISTRY, ANALYTICAL Journal of analytical toxicology Pub Date : 2024-10-28 DOI:10.1093/jat/bkae068

Gregory G Sarris, Dustin L Abbott, Tiffany M Moreno, Kelly J Maychack, Jennifer F Limoges

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Abstract

A simple and rapid qualitative chromatographic method with a unique extraction approach was developed and validated to screen oral fluid samples for 31 compounds in driving under the influence of drugs investigations. The scope and sensitivity of the method meets or exceeds Tier I recommendations established by the National Safety Council's Alcohol, Drugs and Impairment Division. Since this is a targeted chromatographic screen (rather than an immunoassay), cutoffs were set to match the confirmation levels in the recommendations. Sample preparation involved a single-step liquid-liquid extraction procedure, using a mixture of methyl tert-butyl ether, isopropanol, and hexane and was applied to samples collected with the Quantisal™ device. Instrument analysis was conducted by liquid chromatography-tandem mass spectrometry, using a Restek Raptor™ biphenyl column for chromatographic separations and a total run time of 8 min. Validation results met all requirements of ANSI/ASB Standard 036 (1st edition)-Standard Practices for Method Validation in Forensic Toxicology.

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开发和验证一种简单的色谱法，用于在 DUID 调查中筛查口腔液样本中的药物。

开发并验证了一种简单快速的色谱定性方法，该方法采用独特的提取方法，可在药物影响下驾驶调查中筛查口腔液样本中的 31 种化合物。该方法的检测范围和灵敏度达到或超过了美国国家安全委员会酒精、药物和损害分部提出的一级建议。由于这是一种有针对性的色谱筛查（而非免疫测定），因此设置的临界值与建议中的确认水平一致。样品制备采用单步液液萃取法，使用甲基叔丁基醚、异丙醇和正己烷的混合物，适用于使用 QuantisalTM 设备采集的样品。仪器分析采用液相色谱-串联质谱法，使用 Restek RaptorTM 联苯色谱柱进行色谱分离，总运行时间为 8 分钟。验证结果符合 ANSI/ASB 标准 036（第 1 版）--《法医毒理学方法验证标准操作规范》的所有要求。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of analytical toxicology 医学-毒理学

CiteScore

5.10

自引率

20.00%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of Analytical Toxicology (JAT) is an international toxicology journal devoted to the timely dissemination of scientific communications concerning potentially toxic substances and drug identification, isolation, and quantitation. Since its inception in 1977, the Journal of Analytical Toxicology has striven to present state-of-the-art techniques used in toxicology labs. The peer-review process provided by the distinguished members of the Editorial Advisory Board ensures the high-quality and integrity of articles published in the Journal of Analytical Toxicology. Timely presentation of the latest toxicology developments is ensured through Technical Notes, Case Reports, and Letters to the Editor.