Biophysical characterization of hydrogen sulfide: A fundamental exploration in understanding significance in cell signaling

IF 3.3 3区 生物学 Q2 BIOCHEMISTRY & MOLECULAR BIOLOGY Biophysical chemistry Pub Date : 2024-08-30 DOI:10.1016/j.bpc.2024.107317
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Abstract

Hydrogen sulfide (H₂S) has emerged as a significant signaling molecule involved in various physiological processes, including vasodilation, neurotransmission, and cytoprotection. Its interactions with biomolecules are critical to understand its roles in health and disease. Recent advances in biophysical characterization techniques have shed light on the complex interactions of H₂S with proteins, nucleic acids, and lipids. Proteins are primary targets for H₂S, which can modify cysteine residues through S-sulfhydration, impacting protein function and signaling pathways. Advanced spectroscopic techniques, such as mass spectrometry and NMR, have enabled the identification of specific sulfhydrated sites and provided insights into the structural and functional consequences of these modifications. Nucleic acids also interact with H₂S, although this area is less explored compared to proteins. Recent studies have demonstrated that H₂S can induce modifications in nucleic acids, affecting gene expression and stability. Techniques like gel electrophoresis and fluorescence spectroscopy have been utilized to investigate these interactions, revealing that H₂S can protect DNA from oxidative damage and modulate RNA stability and function. Lipids, being integral components of cell membranes, interact with H₂S, influencing membrane fluidity and signaling. Biophysical techniques such as electron paramagnetic resonance (EPR) and fluorescence microscopy have elucidated the effects of H₂S on lipid membranes. These studies have shown that H₂S can alter lipid packing and dynamics, which may impact membrane-associated signaling pathways and cellular responses to stress. In the current work we have integrated this with key scientific explainations to provide a comprehensive review.

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硫化氢的生物物理特征:了解细胞信号传递意义的基础性探索
硫化氢(H₂S)已成为一种重要的信号分子,参与各种生理过程,包括血管扩张、神经传递和细胞保护。它与生物大分子的相互作用对于了解其在健康和疾病中的作用至关重要。生物物理表征技术的最新进展揭示了 H₂S 与蛋白质、核酸和脂质之间复杂的相互作用。蛋白质是 H₂S 的主要作用目标,H₂S 可通过 S-硫化作用改变半胱氨酸残基,从而影响蛋白质的功能和信号传导途径。先进的光谱技术,如质谱法和核磁共振法,能够确定特定的硫氢化位点,并深入了解这些修饰的结构和功能后果。核酸也会与 H₂S 相互作用,但与蛋白质相比,这一领域的研究较少。最新研究表明,H₂S 可诱导核酸发生修饰,从而影响基因的表达和稳定性。研究人员利用凝胶电泳和荧光光谱等技术来研究这些相互作用,发现 H₂S 可以保护 DNA 免受氧化损伤,并调节 RNA 的稳定性和功能。脂质作为细胞膜不可或缺的组成部分,与 H₂S 相互作用,影响膜的流动性和信号传递。电子顺磁共振(EPR)和荧光显微镜等生物物理技术已经阐明了 H₂S 对脂质膜的影响。这些研究表明,H₂S 可以改变脂质的堆积和动态,从而可能影响膜相关的信号传导途径和细胞对应激的反应。在目前的工作中,我们将这些研究与关键的科学解释结合起来,提供了一份全面的综述。
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来源期刊
Biophysical chemistry
Biophysical chemistry 生物-生化与分子生物学
CiteScore
6.10
自引率
10.50%
发文量
121
审稿时长
20 days
期刊介绍: Biophysical Chemistry publishes original work and reviews in the areas of chemistry and physics directly impacting biological phenomena. Quantitative analysis of the properties of biological macromolecules, biologically active molecules, macromolecular assemblies and cell components in terms of kinetics, thermodynamics, spatio-temporal organization, NMR and X-ray structural biology, as well as single-molecule detection represent a major focus of the journal. Theoretical and computational treatments of biomacromolecular systems, macromolecular interactions, regulatory control and systems biology are also of interest to the journal.
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