Ayurveda Detoxification Process Reduces Plumbagin from the Roots of Plumbago zeylanica L. - A RP-UFLC Analysis.

IF 1.5 4区化学 Q4 BIOCHEMICAL RESEARCH METHODS Journal of chromatographic science Pub Date : 2024-09-06 DOI:10.1093/chromsci/bmae048

Gireesh M Ankad, Harsha Hegde, Iranna B Kotturshetti

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Abstract

Ayurveda describes purification process of certain herbal drugs to reduce the toxicity and make them suitable for therapeutic purpose. The objective of the study was to evaluate the effect of detoxification process on plumbagin (PG) from the Plumbago zeylanica L. roots in marketed samples. It involved procurement of market samples from five states of India viz. Andhra Pradesh, Gujarat, Maharashtra, Madhya Pradesh and Punjab. The roots were purified in lime water (LW) as mentioned in Ayurveda. Reverse Phase Ultra-Flow Liquid Chromatography method was validated for identification of PG in unprocessed and processed roots and in the media (LW) used for purification after processing. The data was statistically analyzed by Analysis of Variance (ANOVA) and tested for significance by the Dunnett multiple comparison test. Results were expressed as mean ± SD mg/g dry weight of extract. The study indicated that the PG was reduced quantitatively after processing, while the amount of PG found in the LW was observed to be increased, indicating the leaching of PG during the purification process. In conclusion, the detoxification process eliminates PG from its roots and discloses the leaching effect in the media for the first time.

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阿育吠陀排毒过程减少了 Plumbago zeylanica L. 根中的 Plumbagin - RP-UFLC 分析。

阿育吠陀描述了某些草药的提纯过程，以降低毒性并使其适合治疗目的。这项研究的目的是评估解毒过程对市场样品中 Plumbago zeylanica L. 根中的 plumbagin (PG) 的影响。研究涉及从印度的五个邦（即安得拉邦、古吉拉特邦、马哈拉施特拉邦、中央邦和旁遮普邦）采购市场样品。按照阿育吠陀中的说法，根茎在石灰水（LW）中得到净化。对反相超流液相色谱法进行了验证，以鉴定未加工和加工根茎中的 PG，以及加工后用于净化的介质（石灰水）中的 PG。数据通过方差分析（ANOVA）进行统计分析，并通过邓尼特多重比较检验进行显著性检验。结果以平均值 ± SD 毫克/克提取物干重表示。研究表明，加工后 PG 的数量减少了，而观察到 LW 中 PG 的数量增加了，这表明在提纯过程中 PG 被浸出了。总之，解毒过程消除了根中的 PG，并首次揭示了介质中的浸出效应。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of chromatographic science 化学-分析化学

CiteScore

2.90

自引率

7.70%

发文量

审稿时长

5.6 months

期刊介绍： The Journal of Chromatographic Science is devoted to the dissemination of information concerning all methods of chromatographic analysis. The standard manuscript is a description of recent original research that covers any or all phases of a specific separation problem, principle, or method. Manuscripts which have a high degree of novelty and fundamental significance to the field of separation science are particularly encouraged. It is expected the authors will clearly state in the Introduction how their method compares in some markedly new and improved way to previous published related methods. Analytical performance characteristics of new methods including sensitivity, tested limits of detection or quantification, accuracy, precision, and specificity should be provided. Manuscripts which describe a straightforward extension of a known analytical method or an application to a previously analyzed and/or uncomplicated sample matrix will not normally be reviewed favorably. Manuscripts in which mass spectrometry is the dominant analytical method and chromatography is of marked secondary importance may be declined.