Protein Expression Autoinduction in a Cold-Shock Expression System in Escherichia coli.

Abstract

The cold-shock expression system in Escherichia coli was developed on a manual induction approach using optical density at 600 nm (OD₆₀₀) measurements and isopropyl β-D-1-thiogalactopyranoside (IPTG) addition. In this study, we show that cold-shock expression performs equally well using an autoinduction approach wherein OD₆₀₀ measurements and IPTG addition may be eliminated. We further demonstrate that cold-shock expression with autoinduction can better facilitate high-throughput experiments.