Five miRNAs identified in fucosylated extracellular vesicles as non-invasive diagnostic signatures for hepatocellular carcinoma.

IF 11.7 1区 医学 Q1 CELL BIOLOGY Cell Reports Medicine Pub Date : 2024-09-17 Epub Date: 2024-09-05 DOI:10.1016/j.xcrm.2024.101716
Boan Li, Kun Hao, Mengyang Li, Ailan Wang, Huixue Tang, Lida Xu, Cuidie Ma, Wenqian Du, Lijuan Sun, Xufeng Hou, Tianye Jia, Aixia Liu, Qi Gao, Zhiming Zhao, Ronghua Jin, Ruifu Yang
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Abstract

Hepatocellular carcinoma (HCC) is a prevalent and aggressive cancer that presents significant challenges for early detection. This study introduces the GlyExo-Capture method for isolating fucosylated extracellular vesicles (Fu-EVs) from serum. We analyze microRNA (miRNA) profiles from Fu-EVs in 88 HCC patients and 179 non-HCC controls using next-generation sequencing (NGS) and identify five miRNAs (hsa-let-7a, hsa-miR-21, hsa-miR-125a, hsa-miR-200a, and hsa-miR-150) as biomarkers for HCC diagnosis. The five-miRNA panel demonstrates exceptional HCC diagnostic performance, with a sensitivity of 0.90 and specificity of 0.92 in a combined cohort of 194 HCC and 412 non-HCC controls, significantly surpassing the performance of alpha-fetoprotein (AFP) and des-gamma-carboxy prothrombin (DCP). Notably, the miRNA model achieves recall rates of 85.7% and 90.8% for stage 0 and stage A early-stage HCC, respectively, identifies 88.1% of AFP-negative HCC cases, and effectively differentiates HCC from other cancers. This study provides a high-throughput, rapid, and non-invasive approach for early HCC detection.

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在岩藻糖基化细胞外囊泡中发现的五种 miRNA 是肝细胞癌的非侵入性诊断特征。
肝细胞癌(HCC)是一种常见的侵袭性癌症,给早期检测带来了巨大挑战。本研究介绍了从血清中分离岩藻糖基化细胞外囊泡 (Fu-EVs) 的 GlyExo-Capture 方法。我们利用新一代测序技术(NGS)分析了 88 例 HCC 患者和 179 例非 HCC 对照组的 Fu-EVs 中的 microRNA (miRNA) 图谱,并确定了五种 miRNA(hsa-let-7a、hsa-miR-21、hsa-miR-125a、hsa-miR-200a 和 hsa-miR-150)作为诊断 HCC 的生物标记物。在194例HCC和412例非HCC对照组的联合队列中,五种miRNA面板显示出卓越的HCC诊断性能,灵敏度为0.90,特异性为0.92,大大超过了甲胎蛋白(AFP)和去γ-羧基凝血酶原(DCP)。值得注意的是,该 miRNA 模型对 0 期和 A 期早期 HCC 的召回率分别达到 85.7% 和 90.8%,能识别 88.1% 甲胎蛋白阴性的 HCC 病例,并能有效区分 HCC 和其他癌症。这项研究为早期 HCC 检测提供了一种高通量、快速、无创的方法。
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来源期刊
Cell Reports Medicine
Cell Reports Medicine Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
15.00
自引率
1.40%
发文量
231
审稿时长
40 days
期刊介绍: Cell Reports Medicine is an esteemed open-access journal by Cell Press that publishes groundbreaking research in translational and clinical biomedical sciences, influencing human health and medicine. Our journal ensures wide visibility and accessibility, reaching scientists and clinicians across various medical disciplines. We publish original research that spans from intriguing human biology concepts to all aspects of clinical work. We encourage submissions that introduce innovative ideas, forging new paths in clinical research and practice. We also welcome studies that provide vital information, enhancing our understanding of current standards of care in diagnosis, treatment, and prognosis. This encompasses translational studies, clinical trials (including long-term follow-ups), genomics, biomarker discovery, and technological advancements that contribute to diagnostics, treatment, and healthcare. Additionally, studies based on vertebrate model organisms are within the scope of the journal, as long as they directly relate to human health and disease.
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