Characterisation of the erythrocyte invasion phenotype of FCB-2: A South American P. falciparum reference strain

IF 2.1 3区 医学 Q2 PARASITOLOGY Acta tropica Pub Date : 2024-09-06 DOI:10.1016/j.actatropica.2024.107379
Monica Ararat-Sarria , Hernando Curtidor , Manuel Alfonso Patarroyo
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Abstract

The extent of parasite adaptive capability involved in erythrocyte invasion represents a significant challenge for the development of a Plasmodium falciparum vaccine. The parasite's geographical and populational origin may influence such adaptive behaviour; in vitro culture-adapted parasite strains are typically used for such studies. Previous studies have reported invasion phenotypes in strains from Africa and Asia and, to a lesser extent, from Latin America. This study was aimed at expanding the pool of characterised parasite strains from Latin America by describing the invasion phenotype of the P. falciparum Colombia Bogotá 2 (FCB2) strain. The FCB2 genome was sequenced and erythrocyte invasion ligand sequences were analysed and compared to other previously reported ones. RT-PCR was used for assessing Pfeba family erythrocyte invasion ligands and reticulocyte binding homologue (Pfrh) gene transcription. A flow cytometry-based erythrocyte invasion assay (using enzymatically-treated erythrocytes) was used for determining the FCB2 strain's invasion phenotype. The P. falciparum FCB2 genome sequence was analysed, bearing in mind that prolonged in vitro parasite culture may affect its genome sequence and, in some cases, lead to the deletion of certain genes; it was demonstrated that all erythrocyte invasion ligand gene sequences studied here were preserved. Comparative analysis showed that the target genome sequences were conserved whereas transcriptional analysis highlighted Pfebas and Pfrhs gene expression. Erythrocyte invasion analysis demonstrated that the FCB2 strain has a sialic acid-resistant invasion phenotype.

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南美恶性疟原虫参考菌株 FCB-2 红细胞侵袭表型的特征。
寄生虫在入侵红细胞时的适应能力是开发恶性疟原虫疫苗的一大挑战。寄生虫的地理和种群来源可能会影响这种适应行为;体外培养适应的寄生虫菌株通常用于此类研究。以前的研究报告了来自非洲和亚洲的菌株的入侵表型,其次是来自拉丁美洲的菌株。本研究旨在通过描述恶性疟原虫哥伦比亚波哥大 2 号(FCB2)菌株的入侵表型,扩大拉丁美洲特征化寄生虫菌株库。对 FCB2 基因组进行了测序,分析了红细胞侵袭配体序列,并将其与之前报道的其他配体序列进行了比较。利用 RT-PCR 技术评估了 Pfeba 家族红细胞侵袭配体和网状细胞结合同源物(Pfrh)基因转录情况。流式细胞术红细胞侵袭试验(使用酶处理过的红细胞)用于确定 FCB2 株系的侵袭表型。对恶性疟原虫 FCB2 基因组序列进行了分析,同时考虑到长期体外培养寄生虫可能会影响其基因组序列,并在某些情况下导致某些基因的缺失;结果表明,本文研究的所有红细胞侵袭配体基因序列都得到了保留。比较分析表明,目标基因组序列保持不变,而转录分析则突出了 Pfebas 和 Pfrhs 基因的表达。红细胞侵袭分析表明,FCB2 菌株具有抗唾液酸侵袭表型。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Acta tropica
Acta tropica 医学-寄生虫学
CiteScore
5.40
自引率
11.10%
发文量
383
审稿时长
37 days
期刊介绍: Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.
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