{"title":"Scarless genome editing technology and its application to crop improvement.","authors":"Kazuya Ikeda","doi":"10.1270/jsbbs.23045","DOIUrl":null,"url":null,"abstract":"<p><p>The advent of CRISPR/Cas9 has had a disruptive impact on the world by bringing about dramatic progress and rapid penetration of genome editing technology. However, even though gene disruption can be easily achieved, there has been a challenge in freely changing the sequence. To solve this problem, various novel technologies have emerged in recent years to realize free rewriting of genome sequences. In this review, scarless editing by two-step HDR, a technology that can freely rewrite genomes from a single nucleotide to more than several thousand nucleotides, will be introduced.</p>","PeriodicalId":9258,"journal":{"name":"Breeding Science","volume":null,"pages":null},"PeriodicalIF":2.0000,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11375429/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Breeding Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1270/jsbbs.23045","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/3/9 0:00:00","PubModel":"Epub","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
Abstract
The advent of CRISPR/Cas9 has had a disruptive impact on the world by bringing about dramatic progress and rapid penetration of genome editing technology. However, even though gene disruption can be easily achieved, there has been a challenge in freely changing the sequence. To solve this problem, various novel technologies have emerged in recent years to realize free rewriting of genome sequences. In this review, scarless editing by two-step HDR, a technology that can freely rewrite genomes from a single nucleotide to more than several thousand nucleotides, will be introduced.
期刊介绍：
Breeding Science is published by the Japanese Society of Breeding. Breeding Science publishes research papers, notes and reviews
related to breeding. Research Papers are standard original articles.
Notes report new cultivars, breeding lines, germplasms, genetic
stocks, mapping populations, database, software, and techniques
significant and useful for breeding. Reviews summarize recent and
historical events related breeding.
Manuscripts should be submitted by corresponding author. Corresponding author must have obtained permission from all authors
prior to submission. Correspondence, proofs, and charges of excess page and color figures should be handled by the corresponding author.
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