Sensitivity Analysis of C. auris, S. cerevisiae, and C. cladosporioides by Irradiation with Far-UVC, UVC, and UVB.

Q1 Medicine Pathogens and Immunity Pub Date : 2024-08-21 eCollection Date: 2024-01-01 DOI:10.20411/pai.v9i2.723

Anna-Maria Gierke, Martin Hessling

{"title":"Sensitivity Analysis of C. auris, S. cerevisiae, and C. cladosporioides by Irradiation with Far-UVC, UVC, and UVB.","authors":"Anna-Maria Gierke, Martin Hessling","doi":"10.20411/pai.v9i2.723","DOIUrl":null,"url":null,"abstract":"Background: The World Health Organization has published a list of pathogenic fungi with prior-itizing groups and calls for research and development of antifungal measures, with Candida auris belonging to the group with high priority.Methods: The photosensitivity towards short wavelength ultraviolet irradiation (Far-UVC, UVC, and UVB) was investigated and compared to other yeasts (Saccharomyces cerevisiae) and a mold (Cladosporium cladosporioides). The observed 1-log reduction doses were compared to literature values of other representatives of the genus Candida, but also with S. cerevisiae, Aspergillus niger, and A. fumigatus.Results: For the determined 1-log reduction doses, an increase with higher wavelengths was observed. A 1-log reduction dose of 4.3 mJ/cm2 was determined for C. auris when irradiated at 222 nm, a dose of 6.1 mJ/cm2 at 254 nm and a 1-log reduction dose of 51.3 mJ/cm2 was required when irradiated with UVB.Conclusions: It was observed that S. cerevisiae is a possible surrogate for C. auris for irradiation with Far-UVC and UVB due to close 1-log reduction doses. No surrogate suitability was verified for C. cladosporioides in relation to A. niger and A. fumigatus for irradiation with a wavelength of 254 nm and for A. niger at 222 nm.","PeriodicalId":36419,"journal":{"name":"Pathogens and Immunity","volume":"9 2","pages":"135-151"},"PeriodicalIF":0.0000,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11378758/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Pathogens and Immunity","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.20411/pai.v9i2.723","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/1/1 0:00:00","PubModel":"eCollection","JCR":"Q1","JCRName":"Medicine","Score":null,"Total":0}

引用次数: 0

Abstract

Background: The World Health Organization has published a list of pathogenic fungi with prior-itizing groups and calls for research and development of antifungal measures, with Candida auris belonging to the group with high priority.

Methods: The photosensitivity towards short wavelength ultraviolet irradiation (Far-UVC, UVC, and UVB) was investigated and compared to other yeasts (Saccharomyces cerevisiae) and a mold (Cladosporium cladosporioides). The observed 1-log reduction doses were compared to literature values of other representatives of the genus Candida, but also with S. cerevisiae, Aspergillus niger, and A. fumigatus.

Results: For the determined 1-log reduction doses, an increase with higher wavelengths was observed. A 1-log reduction dose of 4.3 mJ/cm² was determined for C. auris when irradiated at 222 nm, a dose of 6.1 mJ/cm² at 254 nm and a 1-log reduction dose of 51.3 mJ/cm² was required when irradiated with UVB.

Conclusions: It was observed that S. cerevisiae is a possible surrogate for C. auris for irradiation with Far-UVC and UVB due to close 1-log reduction doses. No surrogate suitability was verified for C. cladosporioides in relation to A. niger and A. fumigatus for irradiation with a wavelength of 254 nm and for A. niger at 222 nm.

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

用远紫外线、紫外线和紫外线辐照 C. auris、S. cerevisiae 和 C. cladosporioides 的敏感性分析。

背景：世界卫生组织公布了一份致病真菌优先群体名单，呼吁研究和开发抗真菌措施，白色念珠菌属于优先群体：方法：研究了白色念珠菌对短波紫外线照射（远紫外线、紫外线和紫外线）的光敏性，并与其他酵母菌（酿酒酵母）和霉菌（Cladosporium cladosporioides）进行了比较。观察到的 1-log降低剂量与其他念珠菌属代表菌种的文献值进行了比较，同时也与酿酒酵母、黑曲霉和烟曲霉进行了比较：结果：在确定的 1-log还原剂量中，观察到波长越高，剂量越大。在 222 纳米波长下照射 C. auris，1-log 减少剂量为 4.3 mJ/cm2；在 254 纳米波长下照射 C. auris，1-log 减少剂量为 6.1 mJ/cm2；在 UVB 波长下照射 C. auris，1-log 减少剂量为 51.3 mJ/cm2：结论：研究发现，由于远紫外和紫外线的1-log减少剂量接近，谷氨酸酵母菌可能是用远紫外和紫外线照射C.在波长为 254 纳米和 222 纳米的辐照条件下，C. cladosporioides 与 A. niger 和 A. fumigatus 的代用性未得到验证。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助