Comparative analysis of bile acid composition and metabolism in the liver of Bufo gargarizans aquatic larvae and terrestrial adults

IF 2.2 2区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Comparative Biochemistry and Physiology D-Genomics & Proteomics Pub Date : 2024-09-07 DOI:10.1016/j.cbd.2024.101322

Kaiyue Li , Yufei Wang , Xinyi Li , Hongyuan Wang

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Abstract

Bile acids are crucial for lipid metabolism and their composition and metabolism differ among species. However, there have been no data on the differences in the composition and metabolism of bile acids between aquatic larvae and terrestrial adults of amphibians. This study explored the differences in composition and metabolism of bile acid between Bufo gargarizans larvae and adults. The results demonstrated that adult liver had a lower total bile acid level and a higher conjugated/total bile acid ratio than larval liver. Meanwhile, histological analysis revealed that the larvae showed a larger cross-sectional area of bile canaliculi lumen compared with the adults. The transcriptomic analysis showed that B. gargarizans larvae synthesized bile acids through both the alternative and the 24-hydroxylase pathway, while adults only synthesized bile acids through the 24-hydroxylase pathway. Moreover, bile acid regulator-related genes FXR and RXRα were highly expressed in adult, whereas genes involved in bile acid synthesis (CYP27A1 and CYP46A1) were highly expressed in larvae. The present study will provide valuable insights into understanding metabolic disorders and exploring novel bile acid-based therapeutics.

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水生幼虫和陆生成虫肝脏中胆汁酸组成和代谢的比较分析

胆汁酸对脂质代谢至关重要，不同物种的胆汁酸组成和代谢也不尽相同。然而，目前还没有关于两栖动物水生幼体和陆生成体胆汁酸组成和代谢差异的数据。本研究探讨了蟾蜍幼体和成体胆汁酸组成和代谢的差异。结果表明，与幼体肝脏相比，成体肝脏的总胆汁酸水平较低，共轭胆汁酸/总胆汁酸比率较高。同时，组织学分析表明，幼虫的胆管腔横截面积比成虫大。转录组分析表明，豚鼠幼虫通过替代酶和24-羟化酶两种途径合成胆汁酸，而成虫仅通过24-羟化酶途径合成胆汁酸。此外，胆汁酸调节因子相关基因 FXR 和 RXRα 在成虫中高表达，而参与胆汁酸合成的基因（CYP27A1 和 CYP46A1）在幼虫中高表达。本研究将为了解代谢紊乱和探索基于胆汁酸的新型疗法提供有价值的见解。

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来源期刊

Comparative Biochemistry and Physiology D-Genomics & Proteomics 生物-生化与分子生物学

CiteScore

5.10

自引率

3.30%

发文量

审稿时长

33 days

期刊介绍： Comparative Biochemistry & Physiology (CBP) publishes papers in comparative, environmental and evolutionary physiology. Part D: Genomics and Proteomics (CBPD), focuses on “omics” approaches to physiology, including comparative and functional genomics, metagenomics, transcriptomics, proteomics, metabolomics, and lipidomics. Most studies employ “omics” and/or system biology to test specific hypotheses about molecular and biochemical mechanisms underlying physiological responses to the environment. We encourage papers that address fundamental questions in comparative physiology and biochemistry rather than studies with a focus that is purely technical, methodological or descriptive in nature.