{"title":"Anti-inflammatory 3,4-seco-triterpenoids from Ganoderma cochlear","authors":"","doi":"10.1016/j.tet.2024.134240","DOIUrl":null,"url":null,"abstract":"<div><p>Five new 3,4-<em>seco</em>-lanostane triterpenoids, named ganocochnoids A−E (1−5), along with three known analogues (6−8), were isolated from <em>Ganoderma cochlear</em>. The structures of these new compounds, including their absolute configurations, were characterized by 1D and 2D nuclear magnetic resonance (NMR), computational methods, and HRESIMS. In particular, the structure of compound 1 was confirmed through X-ray crystallographic analysis. The anti-inflammatory activity of all the compounds was evaluated in LPS-induced RAW264.7 cells. The Western blot assay indicated that compounds <strong>4</strong> and <strong>5</strong> were found to have inhibitory effects on iNOS protein expression as well as on mRNA levels of <em>TNF-a</em>, <em>Il-1b</em>, and <em>Il-6</em>, and compound <strong>4</strong> also reduced COX-2 protein expression in LPS-induced RAW264.7 cells.</p></div>","PeriodicalId":437,"journal":{"name":"Tetrahedron","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Tetrahedron","FirstCategoryId":"92","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0040402024004204","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ORGANIC","Score":null,"Total":0}
引用次数: 0
Abstract
Five new 3,4-seco-lanostane triterpenoids, named ganocochnoids A−E (1−5), along with three known analogues (6−8), were isolated from Ganoderma cochlear. The structures of these new compounds, including their absolute configurations, were characterized by 1D and 2D nuclear magnetic resonance (NMR), computational methods, and HRESIMS. In particular, the structure of compound 1 was confirmed through X-ray crystallographic analysis. The anti-inflammatory activity of all the compounds was evaluated in LPS-induced RAW264.7 cells. The Western blot assay indicated that compounds 4 and 5 were found to have inhibitory effects on iNOS protein expression as well as on mRNA levels of TNF-a, Il-1b, and Il-6, and compound 4 also reduced COX-2 protein expression in LPS-induced RAW264.7 cells.
期刊介绍：
Tetrahedron publishes full accounts of research having outstanding significance in the broad field of organic chemistry and its related disciplines, such as organic materials and bio-organic chemistry.
Regular papers in Tetrahedron are expected to represent detailed accounts of an original study having substantially greater scope and details than that found in a communication, as published in Tetrahedron Letters.
Tetrahedron also publishes thematic collections of papers as special issues and ''Reports'', commissioned in-depth reviews providing a comprehensive overview of a research area.
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