Triatomine (Hemiptera: Reduviidae) populations and Trypanosoma cruzi genotyping in peridomestic and sylvatic environments in the semiarid region of Sergipe, Northeastern, Brazil

IF 2.1 3区 医学 Q2 PARASITOLOGY Acta tropica Pub Date : 2024-09-07 DOI:10.1016/j.actatropica.2024.107385
Felipe Mendes Fontes , Jader de Oliveira , André Luiz Rodrigues Menezes , Marta Maria Geraldes Teixeira , David Campos Andrade , João Aristeu da Rosa , Rubens Riscala Madi , Cláudia Moura de Melo
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Abstract

We assessed the diversity of triatomines, the rates of natural infection, and the discrete typing units (DTUs) of Trypanosoma cruzi isolated from them in two municipalities in the state of Sergipe, Brazil. Active searches for triatomines were conducted in the peridomicily and wild enviroments of 10 villages within the two municipalities. Triatomines were taxonomically identified and their feces were extracted using the abdominal compression method. Parasite detection was performed using optical microscopy. For Trypanosoma cruzi genotyping via PCR-FFLB, 151 samples of the subspecies Triatoma brasiliensis macromelasoma and Triatoma brasiliensis were isolated from both municipalities. In total, 505 triatomines were collected, with Triatoma brasiliensis macromelasoma being the most frequent species (58.81 %). Triatoma b. brasiliensis was the only species in both peridomestic and wild environments. Regarding the other species, T. pseudomaculata was found only in the peridomestic environment; and T. b. macromelasoma and Psammolestes tertius were found in the wild environment. Three Discrete Typing Units were identified: TcI (87.51 %) detected in T. b. brasiliensis and T. b. macromelasoma, TcI+TcIII (10.41 %) in T. b. macromelasoma, and TcI+Trypanosoma rangeli (2.08 %) in T. b. macromelasoma. It is concluded that T. b. macromelasoma is the species collected most frequently in the studied region and the one that presents the highest rates of natural infection, highlighting its epidemiological importance for the vectorial transmission of Chagas disease in Sergipe.

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巴西东北部塞尔希培半干旱地区的三足虫(半翅目:Reduviidae)种群和克氏锥虫基因分型
我们在巴西塞尔希培州的两个城市评估了三蠹的多样性、自然感染率以及从它们身上分离出的克鲁斯锥虫的离散分型单位(DTU)。在这两个城市的 10 个村庄的周边环境和野外环境中对三吸虫进行了积极的搜索。对三蠹进行了分类鉴定,并使用腹部压缩法提取了它们的粪便。寄生虫检测采用光学显微镜。在通过 PCR-FFLB 进行克氏锥虫基因分型时,从这两个城市分离出了 151 个大巴西蝽亚种和巴西蝽亚种样本。总共收集到 505 个三蠹样本,其中巴西大三蠹(Triatoma brasiliensis macromelasoma)是最常见的物种(58.81%)。巴西蝽(Triatoma b. brasiliensis)是近郊和野生环境中的唯一物种。至于其他物种,T. pseudomaculata 只出现在家养环境中;T. b. macromelasoma 和 Psammolestes tertius 出现在野生环境中。确定了三个离散分型单元:在巴西疟原虫和大斑疟原虫中检测到 TcI(87.51 %),在大斑疟原虫中检测到 TcI+TcIII (10.41 %),在大斑疟原虫中检测到 TcI+Trypanosoma rangeli (2.08 %)。结论是,T. b. macromelasoma是在研究地区最常采集到的物种,也是自然感染率最高的物种,突出了其在塞尔希培南美锥虫病病媒传播中的流行病学重要性。
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来源期刊
Acta tropica
Acta tropica 医学-寄生虫学
CiteScore
5.40
自引率
11.10%
发文量
383
审稿时长
37 days
期刊介绍: Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.
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