Pentose phosphate pathway inhibition during in vitro maturation substantially affects the metabolism of bovine COCs and blastocysts

IF 2.4 2区 农林科学 Q3 REPRODUCTIVE BIOLOGY Theriogenology Pub Date : 2024-09-10 DOI:10.1016/j.theriogenology.2024.09.009
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Abstract

Glucose metabolism is widely examined in terms of its effect on oocytes and embryos quality. There are two main pathways of glucose metabolism – glycolysis and pentose phosphate pathway (PPP). The glycolytic pathway allows for energy production in the form of ATP and metabolites such as pyruvate and lactate, whereas PPP activity generates NADPH as well as ribose 5-phosphate, a precursor for the synthesis of nucleotides. The aim of the present experiment was the selective inhibition of either glycolysis or PPP during in vitro maturation of bovine cumulus-oocyte complexes (COCs) to demonstrate, how it affects COCs and further embryos with regard to selected lipidomic and metabolomic aspects. Inhibitors of glycolysis (IO) or PPP (DHEA) were applied during IVM, and the control group was matured under standard conditions. A set of COCs from each group was fertilized and obtained embryos were cultured to the blastocyst stage. ATP level was measured in oocytes, relative mRNA level of selected genes involved in energy metabolism was measured in cumulus cells (CC; real time PCR), lipid droplets parameters were evaluated in oocytes and CC whereas metabolome and lipidome (mass spectrometry) were evaluated in oocytes, CC and blastocysts as well. The experiment shows that glycolysis inhibition during IVM affects mainly CC with no effect in oocytes. It allows to maintain the good developmental potential of oocytes and no negative effect of blastocysts quality and quantity is observed. In contrary, PPP inhibition negatively affects metabolic and lipidomic parameters of both oocyte and CC, which further decreases blastocyst rate and quality. It is therefore concluded that PPP is the most crucial pathway of glucose metabolism for COC developmental potential.

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体外成熟过程中磷酸戊糖途径抑制对牛 COC 和囊胚的新陈代谢产生重大影响
葡萄糖代谢对卵母细胞和胚胎质量的影响受到广泛关注。葡萄糖代谢有两种主要途径--糖酵解和磷酸戊糖途径(PPP)。糖酵解途径以 ATP 以及丙酮酸和乳酸等代谢物的形式产生能量,而 PPP 活动则产生 NADPH 以及 5-磷酸核糖(合成核苷酸的前体)。本实验的目的是在牛精原细胞-卵母细胞复合体(COCs)体外成熟过程中选择性地抑制糖酵解或 PPP,以证明其在选定的脂质组学和代谢组学方面如何影响 COCs 及其后的胚胎。在 IVM 期间使用糖酵解抑制剂(IO)或 PPP(DHEA),对照组在标准条件下成熟。对每组的一组 COC 进行受精,获得的胚胎培养至囊胚期。对卵母细胞中的 ATP 水平进行了测量,对积液细胞(CC;实时 PCR)中涉及能量代谢的选定基因的相对 mRNA 水平进行了测量,对卵母细胞和积液细胞中的脂滴参数进行了评估,同时还对卵母细胞、积液细胞和囊胚中的代谢组和脂质组(质谱法)进行了评估。实验表明,IVM 期间的糖酵解抑制主要影响 CC,对卵母细胞没有影响。它能保持卵母细胞良好的发育潜能,对囊胚的质量和数量也没有负面影响。相反,抑制 PPP 会对卵母细胞和 CC 的代谢和脂质体参数产生负面影响,从而进一步降低囊胚率和质量。因此,结论是 PPP 是影响 COC 发育潜力的最关键的葡萄糖代谢途径。
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来源期刊
Theriogenology
Theriogenology 农林科学-生殖生物学
CiteScore
5.50
自引率
14.30%
发文量
387
审稿时长
72 days
期刊介绍: Theriogenology provides an international forum for researchers, clinicians, and industry professionals in animal reproductive biology. This acclaimed journal publishes articles on a wide range of topics in reproductive and developmental biology, of domestic mammal, avian, and aquatic species as well as wild species which are the object of veterinary care in research or conservation programs.
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