Clinical significance of direct fast scarlet staining on the diagnosis of eosinophilic colitis: A comparative study focusing on the eosinophil degranulation in colonic mucosal tissue

IF 2.5 4区医学 Q2 PATHOLOGY Pathology International Pub Date : 2024-09-11 DOI:10.1111/pin.13475

Mina Ikeda, Hiroyuki Kato, Satoshi Arakawa, Takashi Kobayashi, Senju Hashimoto, Yoshiaki Katano, Ken‐ichi Inada, Yuka Kiriyama, Takuma Ishihara, Satoshi Yamamoto, Yukio Asano, Akihiko Horiguchi

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Abstract

This study aimed to validate the DFS (direct fast scarlet) staining in the diagnosis of EC (eosinophilic colitis). The study included 50 patients with EC and 60 with control colons. Among the 60 control samples, 39 and 21 were collected from the ascending and descending colons, respectively. We compared the median number of eosinophils and frequency of eosinophil degranulation by HE (hematoxylin and eosin) and DFS staining between the EC and control groups. In the right hemi‐colon, eosinophil count by HE was useful in distinguishing between EC and control (41.5 vs. 26.0 cells/HPF, p < 0.001), but the ideal cutoff value is 27.5 cells/HPF (high‐power field). However, this method is not useful in the left hemi‐colon (12.5 vs. 13.0 cells/HPF, p = 0.990). The presence of degranulation by DFS allows us to distinguish between the groups even in the left hemi‐colon (58% vs. 5%, p < 0.001). DFS staining also enabled a more accurate determination of degranulation than HE. According to the current standard to diagnose EC (count by HE staining ≥20 cells/HPF), mucosal sampling from left hemi‐colon is problematic since the number of eosinophils could not be increased even in EC. Determination of degranulated eosinophils by DFS may potentiate the diagnostic performance even in such conditions.

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直接快速猩红染色对诊断嗜酸性粒细胞结肠炎的临床意义：以结肠粘膜组织中嗜酸性粒细胞脱颗粒为重点的比较研究

本研究旨在验证 DFS（直接快速猩红染色法）在诊断嗜酸性粒细胞结肠炎（EC）中的有效性。研究对象包括 50 名嗜酸性粒细胞性结肠炎患者和 60 名对照组结肠样本。在 60 份对照样本中，39 份和 21 份分别取自升结肠和降结肠。我们通过 HE（苏木精和伊红）染色和 DFS 染色比较了 EC 组和对照组之间嗜酸性粒细胞的中位数以及嗜酸性粒细胞脱颗粒的频率。在右半结肠，HE 法检测的嗜酸性粒细胞数有助于区分 EC 组和对照组（41.5 个细胞/HPF 对 26.0 个细胞/HPF，p <0.001），但理想的临界值是 27.5 个细胞/HPF（高倍视野）。然而，这种方法在左半结肠（12.5 vs. 13.0 cells/HPF，p = 0.990）中并不适用。即使在左半结肠（58% vs. 5%，p <0.001），DFS 染色法出现的脱颗粒现象也能让我们区分不同组别。与 HE 相比，DFS 染色还能更准确地确定脱颗粒。根据目前诊断EC的标准（HE染色计数≥20个细胞/HPF），从左半结肠粘膜取样是有问题的，因为即使在EC中，嗜酸性粒细胞的数量也不会增加。即使在这种情况下，通过 DFS 检测脱颗粒嗜酸性粒细胞也能提高诊断效果。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Pathology International 医学-病理学

CiteScore

4.50

自引率

4.50%

发文量

102

审稿时长

12 months

期刊介绍： Pathology International is the official English journal of the Japanese Society of Pathology, publishing articles of excellence in human and experimental pathology. The Journal focuses on the morphological study of the disease process and/or mechanisms. For human pathology, morphological investigation receives priority but manuscripts describing the result of any ancillary methods (cellular, chemical, immunological and molecular biological) that complement the morphology are accepted. Manuscript on experimental pathology that approach pathologenesis or mechanisms of disease processes are expected to report on the data obtained from models using cellular, biochemical, molecular biological, animal, immunological or other methods in conjunction with morphology. Manuscripts that report data on laboratory medicine (clinical pathology) without significant morphological contribution are not accepted.