An Advanced Chemometric Model Applied to Ratiometric Surface-Enhanced Raman Scattering for Monitoring Blood Glucose

IF 0.8 4区 化学 Q4 SPECTROSCOPY Journal of Applied Spectroscopy Pub Date : 2024-09-11 DOI:10.1007/s10812-024-01800-1
Y. Tang, S.-W. Zhang, Q. Wang, J.-J. Han, L. Chao, L.-B. Nie, Y. Chen, T. Wang
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Abstract

An advanced chemometric model based on ratiometric surface-enhanced Raman scattering (SERS) was developed for the quantification of glucose in serum samples. In the absence of glucose, it was mainly the SERS signal of silver nanoparticles (AgNPs)-o-phenylenediamine (OPD). When glucose was added, glucose oxidase catalyzed glucose to produce hydrogen peroxide (H2O2), which oxidized OPD to produce 2,3-diaminophenazine (DAP) in the presence of AgNPs. The generated DAP exhibited a new strong SERS signal and changed the Raman peak ratio between DAP and OPD. Without using any internal standard, the advanced chemometric model mitigated the fluctuations in SERS intensity and achieved accurate concentration predictions for glucose in serum samples with recoveries in the ranges of 92.8–104.8%. The accuracy of the quantitative results obtained using the proposed method is comparable with that of the reference method — glucometer. The proposed sensor showed high sensitivity and selectivity in detecting glucose with a limit of detection (LOD) of 0.28 μM. Additionally, the presented SERS sensor demonstrated great promise in determining H2O2-related metabolites in real serum samples.

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用于监测血糖的比率表面增强拉曼散射的高级化学计量模型
研究人员开发了一种基于比率表面增强拉曼散射(SERS)的先进化学计量模型,用于定量检测血清样品中的葡萄糖。在没有葡萄糖的情况下,主要是银纳米粒子(AgNPs)-邻苯二胺(OPD)的 SERS 信号。加入葡萄糖后,葡萄糖氧化酶催化葡萄糖产生过氧化氢(H2O2),在 AgNPs 的存在下,过氧化氢氧化 OPD 生成 2,3-二氨基吩嗪(DAP)。生成的 DAP 显示出新的强 SERS 信号,并改变了 DAP 和 OPD 之间的拉曼峰比。在不使用任何内标物的情况下,先进的化学计量学模型减缓了 SERS 强度的波动,准确预测了血清样品中葡萄糖的浓度,回收率在 92.8-104.8% 之间。使用拟议方法获得的定量结果的准确性与参考方法--血糖仪的准确性相当。所提出的传感器在检测葡萄糖方面具有很高的灵敏度和选择性,检测限(LOD)为 0.28 μM。此外,所提出的 SERS 传感器在测定真实血清样品中与 H2O2 相关的代谢物方面也大有可为。
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来源期刊
CiteScore
1.30
自引率
14.30%
发文量
145
审稿时长
2.5 months
期刊介绍: Journal of Applied Spectroscopy reports on many key applications of spectroscopy in chemistry, physics, metallurgy, and biology. An increasing number of papers focus on the theory of lasers, as well as the tremendous potential for the practical applications of lasers in numerous fields and industries.
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