{"title":"Inter simple sequence repeat (ISSR) based genetic and morphological polymorphism of Azerbaijani grape (Vitis vinifera) genotypes","authors":"Vugar Salimov, Ruhangiz Mammadova, Masum Burak, Shader Alizade, Saida Sharifova, Allah Bakhsh, Nurlan Amrahov, Medina Hamidova","doi":"10.1007/s10722-024-02110-3","DOIUrl":null,"url":null,"abstract":"<p>The assessment of genetic diversity within and between grapevine populations is routinely performed at the molecular level using various laboratory-based techniques. Inter Simple Sequence Repeats (ISSR) markers have been widely used for plant varietal fingerprinting analysis, assessment of hybridization as well as detection of clonal variations and identification of cultivars. The objective of the present study was to reveal the ISSR-based genetic and morphological diversity among different grapevine genotypes, analyse polymorphism level of ISSR primers used, and determine genetic relationship of some Azerbaijani, introduced cultivars and hybrids. Twenty-one cultivars were analysed via ten polymorphic ISSR primers.. Morphological and ampelography data of genotypes studied according OIV descriptors to provide complete information of accessions. ISSR primers produced a total of 56 bands of which 44 were polymorphic. Resolving power of the ISSR primers ranged between 1.61 (UBC 826) and 4.09 (UBC 840). PIC (Polymorphism Information Content) values varied from 0.211(UBC 842) to 0.401 (UBC 810) respectively. The grapevine genotypes were grouped into five major kinship groups. Considering morphological and high genetic similarity value Khalbasar and Apoi Khagog genotypes showed highest genetic similarity index (0.923) and could be theoretically identical. Our results revealed that ISSR genetic markers combined with morphological and ampleography data could be a better tool for evaluation of genetic diversity among the grapevine genotypes.</p>","PeriodicalId":12467,"journal":{"name":"Genetic Resources and Crop Evolution","volume":"20 1","pages":""},"PeriodicalIF":1.6000,"publicationDate":"2024-08-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic Resources and Crop Evolution","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1007/s10722-024-02110-3","RegionNum":3,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"AGRONOMY","Score":null,"Total":0}
引用次数: 0
Abstract
The assessment of genetic diversity within and between grapevine populations is routinely performed at the molecular level using various laboratory-based techniques. Inter Simple Sequence Repeats (ISSR) markers have been widely used for plant varietal fingerprinting analysis, assessment of hybridization as well as detection of clonal variations and identification of cultivars. The objective of the present study was to reveal the ISSR-based genetic and morphological diversity among different grapevine genotypes, analyse polymorphism level of ISSR primers used, and determine genetic relationship of some Azerbaijani, introduced cultivars and hybrids. Twenty-one cultivars were analysed via ten polymorphic ISSR primers.. Morphological and ampelography data of genotypes studied according OIV descriptors to provide complete information of accessions. ISSR primers produced a total of 56 bands of which 44 were polymorphic. Resolving power of the ISSR primers ranged between 1.61 (UBC 826) and 4.09 (UBC 840). PIC (Polymorphism Information Content) values varied from 0.211(UBC 842) to 0.401 (UBC 810) respectively. The grapevine genotypes were grouped into five major kinship groups. Considering morphological and high genetic similarity value Khalbasar and Apoi Khagog genotypes showed highest genetic similarity index (0.923) and could be theoretically identical. Our results revealed that ISSR genetic markers combined with morphological and ampleography data could be a better tool for evaluation of genetic diversity among the grapevine genotypes.
期刊介绍：
Genetic Resources and Crop Evolution is devoted to all aspects of plant genetic resources research. It publishes original articles in the fields of taxonomical, morphological, physiological, biochemical, genetical, cytological or ethnobotanical research of genetic resources and includes contributions to gene-bank management in a broad sense, that means to collecting, maintenance, evaluation, storage and documentation.
Areas of particular interest include:
-crop evolution
-domestication
-crop-weed relationships
-related wild species
-history of cultivated plants including palaeoethnobotany.
Genetic Resources and Crop Evolution also publishes short communications, e.g. newly described crop taxa, nomenclatural notes, reports of collecting missions, evaluation results of gene-bank material etc. as well as book reviews of important publications in the field of genetic resources.
Every volume will contain some review articles on actual problems. The journal is the internationalized continuation of the German periodical Die Kulturpflanze, published formerly by the Institute of Plant Genetics and Crop Plant Research at Gatersleben, Germany.
All contributions are in the English language and are subject to peer reviewing.
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