Phytochemical screening, analgesic, anti-pyretic and antibacterial potentials of Litsea glutinosa (L) leaves extracts in vivo and in vitro technique

Zubair Labu, Samira Karim, Md. Tarekur Rahman, Md. Imran Hossain, Md Shakil
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Abstract

Back ground: Litsea glutinosa leaves (LG) have been used as traditional medicine to treat diseases since ancient times. This study aimed to investigate the cold methanol extract of LG for its analgesic, antipyretic and antibacterial activities, along with preliminary phytochemical screening and acute toxicity test. Methods: In this study, we first investigated the major phytochemicals group present applying modern chromatographic technique HPLC in addition to conventional methods for Phyto screening in cold methanol extracts LG leaves. Both methods simultaneously demonstrated major bioactive compounds found in the extract were phenol, flavonoid. The purposive efficacy and toxicity were then assessed through preclinical testing. Results: In hot plate method, the highest pain inhibitory activity was found at a dose of 500 mg/kg of crude extract (3.37± 0.31sec) which differed significantly (P <0.01 and P <0.001) with that of the standard drug morphine (6.47± 0.23 sec). The extract significantly prolonged reaction latency to thermal-induced pain in hotplate model. Analgesic activity at 500 mg/kg, LG extract produced a 70% suppression of writhing in mice, which was statistically significant (p<0.001) compared to standard morphine's (77.5%) inhibition. In antipyretic activity assay, the crude extract showed notable reduction in body temperature (36.17 ± 0.32°C) at dose of 300 mg/kg-body weight, when the standard (at dose 100 mg/kg-body weight) exerted (36.32 ± 0.67°C) after 3 h of administration. In antibacterial studies, results showed that inhibition of bacterial growth at 400 μg dose of each extract clearly inhibited growth of bacteria from 11 to 22 mm. The extractives carbon tetrachloride fraction, chloroform soluble fraction, ethyl acetate fraction demonstrated notably greater inhibitory zone widths (p < 0.05) against tested strains. Conclusion: Overall, the cold methanol extract of LG leaves demonstrates the therapeutic potential in preclinical settings.
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谷氨酸锂叶(Litsea glutinosa (L))提取物的植物化学筛选、体内和体外镇痛、解热和抗菌潜力技术
背景:谷氨酰麝香草叶(Litsea glutinosa leaves,LG)自古以来就被用作治疗疾病的传统药物。本研究旨在研究 LG 的冷甲醇提取物的镇痛、解热和抗菌活性,同时进行初步的植物化学筛选和急性毒性试验。研究方法在本研究中,我们首先采用现代色谱技术 HPLC 和传统方法对枸杞叶的冷甲醇提取物进行植物化学筛选,以研究其中的主要植物化学物质。这两种方法同时证明了提取物中的主要生物活性化合物是酚类和类黄酮。然后通过临床前试验评估了目的药效和毒性。结果显示在热板法中,粗提物剂量为 500 毫克/千克(3.37± 0.31 秒)时的镇痛活性最高,与标准药物吗啡(6.47± 0.23 秒)相比差异显著(P <0.01 和 P <0.001)。该提取物可明显延长热板模型中热致痛的反应潜伏期。在 500 毫克/千克的镇痛活性中,LG 提取物对小鼠蠕动的抑制率为 70%,与标准吗啡(77.5%)的抑制率相比,具有统计学意义(p<0.001)。在解热活性检测中,粗提取物在 300 毫克/千克体重的剂量下,体温明显降低(36.17 ± 0.32°C),而标准吗啡(剂量为 100 毫克/千克体重)在给药 3 小时后体温为(36.32 ± 0.67°C)。在抗菌研究中,结果表明,每种提取物在 400 μg 剂量下对细菌生长的抑制作用明显,可抑制细菌生长 11 至 22 mm。四氯化碳萃取物、氯仿可溶性萃取物和乙酸乙酯萃取物对测试菌株的抑制区宽度明显更大(p < 0.05)。结论总之,枸杞叶的冷甲醇提取物在临床前研究中具有治疗潜力。
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