Evaluation of C/N ratios (glucose/nitrate) for heterotrophic cultivation of a Brazilian strain of Tetradesmus obliquus: An assessment of heterotrophic, mixotrophic, and photoautotrophic conditions
Renata Augusto Vieira, Marco Shizuo Owatari, Rafael Garcia Lopes, Roberto Bianchini Derner
{"title":"Evaluation of C/N ratios (glucose/nitrate) for heterotrophic cultivation of a Brazilian strain of Tetradesmus obliquus: An assessment of heterotrophic, mixotrophic, and photoautotrophic conditions","authors":"Renata Augusto Vieira, Marco Shizuo Owatari, Rafael Garcia Lopes, Roberto Bianchini Derner","doi":"10.1007/s10811-024-03348-1","DOIUrl":null,"url":null,"abstract":"<p>The growth of microalgae under different trophic modes is widely considered in the literature. However, the cultivation of some microalgal strains under heterotrophic conditions requires further investigation. Thus, the present study investigated the growth potential of a Brazilian strain of <i>Tetradesmus obliquus</i> (Chlorophyceae, Scenedesmaceae) in terms of heterotrophic metabolism. For this purpose, cultures were generated using different concentrations of glucose (26, 13, and 6.5 g L<sup>–1</sup>) and sodium nitrate (0.6 g, 0.3, and 0.15 g L<sup>–1</sup>) in culture media. The growth parameters analysed were the maximum biomass achieved and volumetric productivity. The combination of 26 g L<sup>–1</sup> glucose with 0.6 g L<sup>–1</sup> sodium nitrate resulted in the greatest increase in dry weight (9.63 ± 0.15 g L<sup>–1</sup>). Then, <i>T. obliquus</i> cultures under heterotrophic conditions were compared with those under photoautotrophic and mixotrophic conditions. The maximum production in heterotrophic cultivation was similar to what had been obtained, while mixotrophic cultivation presented the best result, 14.77 ± 0.06 g L<sup>–1</sup>, and photoautotrophic cultivation obtained a maximum biomass of 7.90 ± 0.17 g L<sup>–1</sup>. The productivity values achieved were 0.52 ± 0.04 g L<sup>–1</sup> day<sup>–1</sup>, 0.98 ± 0.07 g L<sup>–1</sup> day<sup>–1</sup>, and 1.24 ± 0.07 g L<sup>–1</sup> day<sup>–1</sup> for photoautotrophic, heterotrophic and mixotrophic cultures, respectively. The results indicated that the Brazilian microalga <i>T. obliquus</i> has the potential to be successfully cultivated heterotrophically.</p><h3 data-test=\"abstract-sub-heading\">Graphical Abstract</h3>\n","PeriodicalId":15086,"journal":{"name":"Journal of Applied Phycology","volume":null,"pages":null},"PeriodicalIF":2.8000,"publicationDate":"2024-09-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Applied Phycology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1007/s10811-024-03348-1","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
The growth of microalgae under different trophic modes is widely considered in the literature. However, the cultivation of some microalgal strains under heterotrophic conditions requires further investigation. Thus, the present study investigated the growth potential of a Brazilian strain of Tetradesmus obliquus (Chlorophyceae, Scenedesmaceae) in terms of heterotrophic metabolism. For this purpose, cultures were generated using different concentrations of glucose (26, 13, and 6.5 g L–1) and sodium nitrate (0.6 g, 0.3, and 0.15 g L–1) in culture media. The growth parameters analysed were the maximum biomass achieved and volumetric productivity. The combination of 26 g L–1 glucose with 0.6 g L–1 sodium nitrate resulted in the greatest increase in dry weight (9.63 ± 0.15 g L–1). Then, T. obliquus cultures under heterotrophic conditions were compared with those under photoautotrophic and mixotrophic conditions. The maximum production in heterotrophic cultivation was similar to what had been obtained, while mixotrophic cultivation presented the best result, 14.77 ± 0.06 g L–1, and photoautotrophic cultivation obtained a maximum biomass of 7.90 ± 0.17 g L–1. The productivity values achieved were 0.52 ± 0.04 g L–1 day–1, 0.98 ± 0.07 g L–1 day–1, and 1.24 ± 0.07 g L–1 day–1 for photoautotrophic, heterotrophic and mixotrophic cultures, respectively. The results indicated that the Brazilian microalga T. obliquus has the potential to be successfully cultivated heterotrophically.
微藻在不同营养模式下的生长在文献中得到广泛关注。然而，一些微藻菌株在异养条件下的培养还需要进一步研究。因此，本研究从异养代谢的角度研究了巴西四裂殖藻（叶绿藻科，Scenedesmaceae）菌株的生长潜力。为此，在培养基中添加了不同浓度的葡萄糖（26、13 和 6.5 g L-1）和硝酸钠（0.6 g、0.3 和 0.15 g L-1）。分析的生长参数是获得的最大生物量和体积生产率。26 g L-1 葡萄糖与 0.6 g L-1 硝酸钠的组合使干重增加最多（9.63 ± 0.15 g L-1）。然后，将异养条件下的斜管蛙培养物与光自养和混养条件下的培养物进行了比较。异养栽培的最大产量与已获得的结果相似，而混养栽培的结果最好，为 14.77 ± 0.06 g L-1，光自养栽培获得的最大生物量为 7.90 ± 0.17 g L-1。光自养、异养和混养的生产率值分别为 0.52 ± 0.04 g L-1 天-1、0.98 ± 0.07 g L-1 天-1 和 1.24 ± 0.07 g L-1 天-1。结果表明，巴西微藻 T. obliquus 具有成功进行异养培养的潜力。
期刊介绍：
The Journal of Applied Phycology publishes work on the rapidly expanding subject of the commercial use of algae.
The journal accepts submissions on fundamental research, development of techniques and practical applications in such areas as algal and cyanobacterial biotechnology and genetic engineering, tissues culture, culture collections, commercially useful micro-algae and their products, mariculture, algalization and soil fertility, pollution and fouling, monitoring, toxicity tests, toxic compounds, antibiotics and other biologically active compounds.
Each issue of the Journal of Applied Phycology also includes a short section for brief notes and general information on new products, patents and company news.
京公网安备 11010802042870号
京ICP备2023020795号-1
分享
求助内容：
应助结果提醒方式：
扫码关注我们
