Antonio Guarnieri, Noemi Venditti, Marco Alfio Cutuli, Natasha Brancazio, Giovanna Salvatore, Irene Magnifico, Laura Pietrangelo, Marilina Falcone, Franca Vergalito, Daria Nicolosi, Franco Scarsella, Sergio Davinelli, Giovanni Scapagnini, Giulio Petronio Petronio, Roberto Di Marco
{"title":"Human breast milk isolated lactic acid bacteria: antimicrobial and immunomodulatory activity on the Galleria mellonella burn wound model","authors":"Antonio Guarnieri, Noemi Venditti, Marco Alfio Cutuli, Natasha Brancazio, Giovanna Salvatore, Irene Magnifico, Laura Pietrangelo, Marilina Falcone, Franca Vergalito, Daria Nicolosi, Franco Scarsella, Sergio Davinelli, Giovanni Scapagnini, Giulio Petronio Petronio, Roberto Di Marco","doi":"10.3389/fcimb.2024.1428525","DOIUrl":null,"url":null,"abstract":"IntroductionManaging burn injuries is a challenge in healthcare. Due to the alarming increase in antibiotic resistance, new prophylactic and therapeutic strategies are being sought. This study aimed to evaluate the potential of live Lactic Acid Bacteria for managing burn infections, using <jats:italic>Galleria mellonella larvae</jats:italic> as an alternative preclinical animal model and comparing the outcomes with a common antibiotic.MethodsThe antimicrobial activity of LAB isolated from human breast milk was assessed <jats:italic>in vitro</jats:italic> against <jats:italic>Pseudomonas aeruginosa</jats:italic> ATCC 27853. Additionally, the immunomodulatory effects of LAB were evaluated <jats:italic>in vivo</jats:italic> using the <jats:italic>G. mellonella</jats:italic> burn wound infection model.Results and discussion<jats:italic>In vitro</jats:italic> results demonstrated the antimicrobial activity of Lactic Acid Bacteria against <jats:italic>P. aeruginosa</jats:italic>. <jats:italic>In vivo</jats:italic> results show that their prophylactic treatment improves, statistically significant, larval survival and modulates the expression of immunity-related genes, Gallerimycin and Relish/NF-κB, strain-dependently. These findings lay the foundation and suggest a promising alternative for burn wound prevention and management, reducing the risk of antibiotic resistance, enhancing immune modulation, and validating the potential <jats:italic>G. mellonella</jats:italic> as a skin burn wound model.","PeriodicalId":12458,"journal":{"name":"Frontiers in Cellular and Infection Microbiology","volume":null,"pages":null},"PeriodicalIF":4.6000,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Frontiers in Cellular and Infection Microbiology","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.3389/fcimb.2024.1428525","RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"IMMUNOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
IntroductionManaging burn injuries is a challenge in healthcare. Due to the alarming increase in antibiotic resistance, new prophylactic and therapeutic strategies are being sought. This study aimed to evaluate the potential of live Lactic Acid Bacteria for managing burn infections, using Galleria mellonella larvae as an alternative preclinical animal model and comparing the outcomes with a common antibiotic.MethodsThe antimicrobial activity of LAB isolated from human breast milk was assessed in vitro against Pseudomonas aeruginosa ATCC 27853. Additionally, the immunomodulatory effects of LAB were evaluated in vivo using the G. mellonella burn wound infection model.Results and discussionIn vitro results demonstrated the antimicrobial activity of Lactic Acid Bacteria against P. aeruginosa. In vivo results show that their prophylactic treatment improves, statistically significant, larval survival and modulates the expression of immunity-related genes, Gallerimycin and Relish/NF-κB, strain-dependently. These findings lay the foundation and suggest a promising alternative for burn wound prevention and management, reducing the risk of antibiotic resistance, enhancing immune modulation, and validating the potential G. mellonella as a skin burn wound model.
导言处理烧伤是医疗保健领域的一项挑战。由于抗生素耐药性的惊人增长，人们正在寻求新的预防和治疗策略。本研究旨在评估活乳酸菌在治疗烧伤感染方面的潜力，使用绿孔雀幼虫作为另一种临床前动物模型，并将其结果与普通抗生素进行比较。结果与讨论体外结果显示乳酸菌对铜绿假单胞菌具有抗菌活性。体内研究结果表明，乳酸菌预防性治疗可显著提高幼虫存活率，并调节免疫相关基因（加勒霉素和Relish/NF-κB）的表达。这些发现为烧伤创面的预防和管理奠定了基础，并提出了一种前景广阔的替代方法，可降低抗生素耐药性的风险，增强免疫调节功能，并验证 G. mellonella 作为皮肤烧伤创面模型的潜力。
期刊介绍：
Frontiers in Cellular and Infection Microbiology is a leading specialty journal, publishing rigorously peer-reviewed research across all pathogenic microorganisms and their interaction with their hosts. Chief Editor Yousef Abu Kwaik, University of Louisville is supported by an outstanding Editorial Board of international experts. This multidisciplinary open-access journal is at the forefront of disseminating and communicating scientific knowledge and impactful discoveries to researchers, academics, clinicians and the public worldwide.
Frontiers in Cellular and Infection Microbiology includes research on bacteria, fungi, parasites, viruses, endosymbionts, prions and all microbial pathogens as well as the microbiota and its effect on health and disease in various hosts. The research approaches include molecular microbiology, cellular microbiology, gene regulation, proteomics, signal transduction, pathogenic evolution, genomics, structural biology, and virulence factors as well as model hosts. Areas of research to counteract infectious agents by the host include the host innate and adaptive immune responses as well as metabolic restrictions to various pathogenic microorganisms, vaccine design and development against various pathogenic microorganisms, and the mechanisms of antibiotic resistance and its countermeasures.
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