ChatGPT as a bioinformatic partner.

Abstract

The advanced Large Language Model ChatGPT4o, developed by OpenAI, can be used in the field of bioinformatics to analyze and understand cross-reactive allergic reactions. This study explores the use of ChatGPT4o to support research on allergens, particularly in the cross-reactivity syndrome between cat and pork. Using a hypothetical clinical case of a child with a confirmed allergy to Fel d 2 (cat albumin) and Sus s 1 (pork albumin), the model guided data collection, protein sequence analysis, and three-dimensional structure visualization. Through the use of bioinformatics tools like SDAP 2.0 and BepiPRED, the epitope regions of the allergenic proteins were predicted, con-firming their accessibility to immunoglobulin E (IgE) and probability of cross-reactivity. The results show that regions with high epitope probability exhibit high surface accessibility and predominantly coil and helical structures. The construction of a phylogenetic tree further sup-ported the evolutionary relationships among the studied allergens. ChatGPT4o has demonstrated its usefulness in guiding non-specialist researchers through complex bioinformatics processes, making advanced science accessible and improving analytical and innovation capabilities.