Bacteria exhibit optimal diffusivity near surfaces

Abstract

In natural environments, solid surfaces present both opportunities and challenges for bacteria. On one hand, they serve as platforms for biofilm formation, crucial for bacterial colonization and resilience in harsh conditions. On the other hand, surfaces can entrap bacteria, constraining their environmental exploration compared to the freedom they experience in bulk liquid. Here, through systematic single-cell behavioral measurements, phenomenological modeling, and theoretical analysis, we reveal how bacteria strategically navigate these factors. We observe that bacterial surface residence time decreases sharply with increasing tumble bias, transitioning to a plateau at a tumble bias of around 0.25, consistent with the mean tumble bias of wild-type Escherichia coli. Furthermore, we find that bacterial surface diffusivity peaks near the mean tumble bias of wild-type E. coli. This reflects a bet-hedging strategy: some bacteria swiftly escape from the surface, while others, with longer surface residence times, explore this two-dimensional environment most efficiently.