Pooja Moni Baruah, Niraj Agarwala, Kuntala Sarma Bordoloi, Preetom Regon, Bhaben Tanti
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引用次数: 0
Abstract
Tea plants exposed to temperature stress conditions exhibit reduced quality and yield. Long non-coding RNAs (lncRNAs) are key regulators in temperature stress responses. A genome-wide lncRNA analysis using RNA sequencing data from tea plants under varying temperature stresses was carried out in this study. The analysis identified a total of 23589 putative lncRNAs, with 2483 being differentially expressed (DE). Weighted gene co-expression network analysis (WGCNA) showed 445 DE lncRNAs co-expressed with 544 genes associated to temperature stress responses. Functional annotation indicated that these genes are involved in processes like protein folding, cellular response to decreased oxygen level, response to hypoxia, unfolded protein binding, and response to heat during high temperature stresses; and response to cold, water transport, and water channel activity during low temperature stresses. Additionally, competing endogenous RNA (ceRNA) network analysis revealed 230 temperature-responsive lncRNAs regulating 400 DE genes via 106 microRNAs (miRNAs). To validate high-throughput sequencing data, primers were designed for eight DE lncRNAs, and their expression levels were confirmed. This study enhances understanding of lncRNAs in temperature stress responses, providing a foundation for further research in tea plants.
期刊介绍:
The Journal of Plant Growth Regulation is an international publication featuring original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research on various aspects of plant growth and development using hormonal, physiological, environmental, genetic, biophysical, developmental and/or molecular approaches.
The journal also publishes timely reviews on highly relevant areas and/or studies in plant growth and development, including interdisciplinary work with an emphasis on plant growth, plant hormones and plant pathology or abiotic stress.
In addition, the journal features occasional thematic issues with special guest editors, as well as brief communications describing novel techniques and meeting reports.
The journal is unlikely to accept manuscripts that are purely descriptive in nature or reports work with simple tissue culture without attempting to investigate the underlying mechanisms of plant growth regulation, those that focus exclusively on microbial communities, or deal with the (elicitation by plant hormones of) synthesis of secondary metabolites.