Physiological Age of Stock Plants Determines Phytohormonal Changes in Leafy Cuttings of Prunus subhirtella 'Autumnalis'

IF 3.9 3区 生物学 Q1 PLANT SCIENCES Journal of Plant Growth Regulation Pub Date : 2024-09-09 DOI:10.1007/s00344-024-11479-5
Petra Kunc, Aljaž Medič, Metka Hudina, Robert Veberič, Gregor Osterc
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Abstract

The aim of this study was to investigate adventitious root formation in cuttings obtained from physiologically different old stock material. The ornamental cherry Prunus subhirtella ´Autumnalis´ was used for the experiment. We examined three stock plants, namely a physiologically mature stock plant (about 60 years old) and physiologically juvenile plants (21 years old), which were previously propagated by cuttings, semi-mature stock material and in vitro juvenile stock material. We also investigated the role of phytohormones in the induction phase of adventitious root (AR) formation depending on the physiological age of the stock plant and the time after cutting. High performance liquid chromatography coupled with mass spectrometry (HPLC–MS/MS) was used to identify and quantify the phytohormones. The difference in rooting and quality of the developed AR is observed between semi-mature stock and mature stock material. Cuttings from semi-mature plants rooted in 95.00% of cases, while cuttings from mature stock plants only rooted in 68.33%. The high concentration of strigolactones and jasmonic acid (JA) immediately after severance had an inhibitory effect on the development of adventitious roots, especially in cuttings of mature origin. The development of AR is positively influenced by the increase in indole-3-acetic acid (IAA) 4 h after cutting from the stock plant. Our results show that the formation of adventitious root formation depends on the concentration and ratio between different phytohormones.

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插条植株的生理年龄决定了亚黑李'Autumnalis'叶插条的植物激素变化
本研究的目的是调查从生理上不同的老树材料中获得的插条中不定根的形成情况。实验使用的是观赏樱桃 Prunus subhirtella ´Autumnalis´ 。我们考察了三种原种植株,即生理成熟的原种植株(约 60 岁)和生理幼苗(21 岁),它们以前都是通过扦插、半成熟原种材料和离体幼苗材料繁殖的。我们还研究了植物激素在不定根(AR)形成诱导阶段的作用,这取决于原种植株的生理年龄和扦插后的时间。我们采用高效液相色谱-质谱联用技术(HPLC-MS/MS)对植物激素进行了鉴定和定量。观察到半成熟植株和成熟植株材料在生根和培养出的 AR 质量方面存在差异。来自半成熟植株的插条有 95.00% 生根,而来自成熟植株的插条只有 68.33% 生根。扦插后立即产生的高浓度绞股蓝内酯和茉莉酸(JA)对不定根的发展有抑制作用,尤其是在成熟插条上。从原种植株扦插 4 小时后,吲哚-3-乙酸(IAA)的增加对 AR 的发育有积极影响。我们的研究结果表明,不定根的形成取决于不同植物激素的浓度和比例。
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来源期刊
CiteScore
8.40
自引率
6.20%
发文量
312
审稿时长
1.8 months
期刊介绍: The Journal of Plant Growth Regulation is an international publication featuring original articles on all aspects of plant growth and development. We welcome manuscripts reporting question-based research on various aspects of plant growth and development using hormonal, physiological, environmental, genetic, biophysical, developmental and/or molecular approaches. The journal also publishes timely reviews on highly relevant areas and/or studies in plant growth and development, including interdisciplinary work with an emphasis on plant growth, plant hormones and plant pathology or abiotic stress. In addition, the journal features occasional thematic issues with special guest editors, as well as brief communications describing novel techniques and meeting reports. The journal is unlikely to accept manuscripts that are purely descriptive in nature or reports work with simple tissue culture without attempting to investigate the underlying mechanisms of plant growth regulation, those that focus exclusively on microbial communities, or deal with the (elicitation by plant hormones of) synthesis of secondary metabolites.
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