Effect of Cuminum cyminum essential oil on growth, histology, intestinal histomorphology, blood parameters, gonadal development, antioxidant activity, and disease resistance against Aeromonas hydrophila in Cyprinus carpio

Abstract

This study investigated the effects on growth performance, intestinal histomorphology, blood parameters, gonadal development, antioxidant activity, and disease resistance against Aeromonas hydrophila of carp fed with cumin. Fish were fed diets containing 0, 1.0, and 3.0 mL kg−1 cumin for 60 days. At the end of the experiment, the use of cumin oil did not affect growth performances and survival rate after challenge with A. hydrophila (p > 0.05). However, villi length and goblet cells increased in carp fed with cumin diets. In addition, no pathological findings were observed in the hepatopancreas, gonad, and intestine tissues of carp fed with cumin. Total protein; albumin; aspartate aminotransferase, alanine aminotransferase, and alkaline phosphatase levels; superoxide dismutase; and catalase activity were similar in cumin oil supplemented groups compared with the control. Both male and female fecundity increased with the addition of 1.0 mL kg−1 cumin, whereas the addition of 3.0 mL kg−1 cumin caused a slight decrease in fertility. As a result, the use of cumin essential oil at 1 mL kg−1 concentration can be recommended to improve fish health and increase fertility in carp culture.