Circular eight-room maze for assessing spatial learning and memory functions in rats: An example using a traumatic brain injury model

Abstract

Background and objectives

This study introduced an animal cognitive function assessment system using a novel circular eight-room maze (CERM). The CERM, designed for tracking path trajectories in animal models of cognitive impairment pathologies such as traumatic brain injury (TBI), comprised a 120-cm diameter disk with eight rooms (30 cm × 25 cm × 30 cm).

Methods

These rooms have magnetic interfaces for modular assembly and disassembly. Notably, one room remained dark and contained food, while the remaining seven rooms automatically lit up when a rat entry, facilitating the assessment of the rat's learning and spatial memory. An infrared night vision camera captured the animal's search trajectory, and binary image processing techniques were employed to eliminate noise and extract the rat's position coordinates to record the rat's path trajectories. The system automatically calculated various cognitive assessment parameters, such as latency, distance traveled, time spent in each quadrant, inner and outer area exploration times, short-term and long-term memory errors, and the number of entries to all the rooms by chance/by memory.

Results

The analysis of overall path trajectories revealed increasingly erratic movement and a growing reliance on chance to enter rooms in rats with TBI over time, likely due to declining memory and the consequent inability to locate the food room. Moreover, increased trajectories in the first quadrant and inner area characterized the behavior of rats with TBI, with statistically significant differences from the sham group observed on day 7. By day 28, all cognitive parameters except short-term memory error significantly differed between the two groups.

Conclusion

Experimental data indicated a substantial increase in irregular search behavior in the TBI group over time, suggesting deterioration in cognitive function and an inability to accurately recall the food room. Conversely, the sham group exhibited consistent search trajectories, typically following the walls and rapidly locating the food room. Moreover, their room entries were guided by memory rather than by chance. Compared with traditional maze tests, this system's strengths lie in its ability to provide more quantitative data and vividly portray behavioral patterns. Therefore, the proposed CERM system can be used as an effective tool for cognitive assessment.