Cytotoxicity and genotoxic impacts of LAPONITE® on murine adipose stem cells

IF 5.3 2区地球科学 Q2 CHEMISTRY, PHYSICAL Applied Clay Science Pub Date : 2024-09-14 DOI:10.1016/j.clay.2024.107572

Francisca L.A. Cardoso , Letícya L.S. Soares , Danielle B.A. Silva , Napoleão M. Argôlo Neto , Edson C. Silva Filho , Adalberto S. Silva , Dayseanny O. Bezerra , Maria S.P. Cruz , Felipe C. Nunes , Wanderson G.G. Melo , João M.C. Sousa , Antonielly C. Reis , Maria A.M. Carvalho

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Abstract

This study comprehensively characterized LAPONITE® and evaluated its cytotoxic and genotoxic effects on rat adipose-derived stem cells (ADSCs). Chemical analyses included Fourier transform infrared spectroscopy, thermogravimetric analysis, X-ray diffraction, and scanning electron microscopy. Cytotoxic screening using the Artemia salina lethality test indicated low toxicity. ADSCs exhibited mesenchymal stem cell chracteristics. LAPONITE® demonstrated osteogenic induction capability. MTT assays revealed increased cell viability across concentrations and exposure times. The comet assay for genotoxicity showed no significant differences in damage index between experimental groups, except for a distinction in the frequency of damage between LAPONITE® concentrations at the longest exposure time. This comprehensive assessment provides valuable insights into the safety and potential applications of LAPONITE® in regenerative medicine.

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LAPONITE® 对小鼠脂肪干细胞的细胞毒性和遗传毒性影响

本研究全面描述了 LAPONITE® 的特性，并评估了其对大鼠脂肪干细胞（ADSCs）的细胞毒性和基因毒性作用。化学分析包括傅立叶变换红外光谱、热重分析、X 射线衍射和扫描电子显微镜。使用盐水蒿致死试验进行的细胞毒性筛选表明毒性很低。ADSCs 具有间充质干细胞特性。LAPONITE® 具有成骨诱导能力。MTT 检测显示，不同浓度和暴露时间的细胞存活率均有所提高。针对遗传毒性的彗星试验表明，除了在最长暴露时间内不同浓度的 LAPONITE® 之间的损伤频率不同外，实验组之间的损伤指数没有明显差异。这项综合评估为 LAPONITE® 在再生医学中的安全性和潜在应用提供了宝贵的见解。

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来源期刊

Applied Clay Science 地学-矿物学

CiteScore

10.30

自引率

10.70%

发文量

289

审稿时长

39 days

期刊介绍： Applied Clay Science aims to be an international journal attracting high quality scientific papers on clays and clay minerals, including research papers, reviews, and technical notes. The journal covers typical subjects of Fundamental and Applied Clay Science such as: • Synthesis and purification • Structural, crystallographic and mineralogical properties of clays and clay minerals • Thermal properties of clays and clay minerals • Physico-chemical properties including i) surface and interface properties; ii) thermodynamic properties; iii) mechanical properties • Interaction with water, with polar and apolar molecules • Colloidal properties and rheology • Adsorption, Intercalation, Ionic exchange • Genesis and deposits of clay minerals • Geology and geochemistry of clays • Modification of clays and clay minerals properties by thermal and physical treatments • Modification by chemical treatments with organic and inorganic molecules(organoclays, pillared clays) • Modification by biological microorganisms. etc...