{"title":"Sweet RNA","authors":"Yiyun Song","doi":"10.1038/s41589-024-01746-7","DOIUrl":null,"url":null,"abstract":"<p>By coupling rPAL to enzymatic glycan cleavage and sequential window acquisition of all theoretical mass spectra (SWATH-MS), the team found that acp<sup>3</sup>U was the most enriched linker (although other putative linker substrates were identified). Importantly, knocking out DTWD2, an enzyme responsible for installing acp<sup>3</sup>U in tRNAs, reduced glycoRNA levels. This study provides not only a useful tool for glycoRNA detection, but also the first direct evidence of a covalent linkage between a secretory N-glycan and RNA, paving the way for future functional studies.</p><p><b>Original reference:</b> <i>Cell</i> https://doi.org/10.1016/j.cell.2024.07.044 (2024)</p>","PeriodicalId":18832,"journal":{"name":"Nature chemical biology","volume":null,"pages":null},"PeriodicalIF":12.9000,"publicationDate":"2024-09-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Nature chemical biology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1038/s41589-024-01746-7","RegionNum":1,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMISTRY & MOLECULAR BIOLOGY","Score":null,"Total":0}
引用次数: 0
Abstract
By coupling rPAL to enzymatic glycan cleavage and sequential window acquisition of all theoretical mass spectra (SWATH-MS), the team found that acp3U was the most enriched linker (although other putative linker substrates were identified). Importantly, knocking out DTWD2, an enzyme responsible for installing acp3U in tRNAs, reduced glycoRNA levels. This study provides not only a useful tool for glycoRNA detection, but also the first direct evidence of a covalent linkage between a secretory N-glycan and RNA, paving the way for future functional studies.
Original reference:Cell https://doi.org/10.1016/j.cell.2024.07.044 (2024)
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