Exogenous interactome analysis of bovine viral diarrhea virus-host using network based-approach and identification of hub genes and important pathways involved in virus pathogenesis

IF 2.3 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY Biochemistry and Biophysics Reports Pub Date : 2024-09-16 DOI:10.1016/j.bbrep.2024.101825
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Abstract

Bovine viral diarrhea (BVD) is one of the most important diseases in livestock, caused by BVD virus (BVDV). During the pathogenesis of the virus, many interactions occur between host and viral proteins. Studying these interactions can help better understand the pathogenesis of the virus, identify putative functional proteins, and find new treatment and prevention strategies. To this aim, a BVDV-host protein-protein interaction (PPI) network map was constructed using Cytoscape and analyzed with cytoHubba, Kyoto Encyclopedia of Genes and Genomics (KEGG), Gene Ontology (GO), and Protein Analysis Through Evolutionary Relationships (PANTHER). Npro with 125 connections had the greatest number of interactions with host proteins. CD46, EEF-2, and TXN genes were detected as hub genes using different ranking algorithms in cytoHubba. BVDV interactions with its host mainly focus on targeting translation, protein synthesis, and cellular metabolism pathways. Different classes of proteins including translational proteins, nucleic acid metabolism proteins, metabolite interconversion enzymes, and protein-modifying enzymes are affected by BVDV. These findings improve our understanding of the effects of the virus on the cell. Hub genes and key pathways identified in the present study can serve as targets for novel BVDV prevention or treatment strategies.

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利用基于网络的方法对牛病毒性腹泻病毒-宿主的外源相互作用组进行分析,并确定参与病毒致病的枢纽基因和重要通路
牛病毒性腹泻(BVD)是家畜最重要的疾病之一,由 BVD 病毒(BVDV)引起。在病毒的致病过程中,宿主蛋白和病毒蛋白之间会发生许多相互作用。研究这些相互作用有助于更好地了解病毒的致病机理,确定假定的功能蛋白,并找到新的治疗和预防策略。为此,研究人员使用Cytoscape构建了BVDV-宿主蛋白质-蛋白质相互作用(PPI)网络图,并使用cytoHubba、京都基因和基因组学百科全书(KEGG)、基因本体(GO)和蛋白质进化关系分析(PANTHER)进行了分析。有 125 个连接的 Npro 与宿主蛋白质相互作用的次数最多。利用 cytoHubba 中的不同排序算法,CD46、EEF-2 和 TXN 基因被检测为枢纽基因。BVDV 与宿主的相互作用主要集中在针对翻译、蛋白质合成和细胞代谢途径。包括翻译蛋白、核酸代谢蛋白、代谢物相互转换酶和蛋白质修饰酶在内的不同类别的蛋白质都受到了BVDV的影响。这些发现加深了我们对病毒对细胞影响的理解。本研究发现的枢纽基因和关键通路可作为新型 BVDV 预防或治疗策略的目标。
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来源期刊
Biochemistry and Biophysics Reports
Biochemistry and Biophysics Reports Biochemistry, Genetics and Molecular Biology-Biophysics
CiteScore
4.60
自引率
0.00%
发文量
191
审稿时长
59 days
期刊介绍: Open access, online only, peer-reviewed international journal in the Life Sciences, established in 2014 Biochemistry and Biophysics Reports (BB Reports) publishes original research in all aspects of Biochemistry, Biophysics and related areas like Molecular and Cell Biology. BB Reports welcomes solid though more preliminary, descriptive and small scale results if they have the potential to stimulate and/or contribute to future research, leading to new insights or hypothesis. Primary criteria for acceptance is that the work is original, scientifically and technically sound and provides valuable knowledge to life sciences research. We strongly believe all results deserve to be published and documented for the advancement of science. BB Reports specifically appreciates receiving reports on: Negative results, Replication studies, Reanalysis of previous datasets.
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