Qualitative analysis of Fasciola gigantica excretory and secretory products coimmunoprecipitated with buffalo secondary infection sera shows dissimilar components from primary infection sera

IF 2.1 3区 医学 Q2 PARASITOLOGY Acta tropica Pub Date : 2024-09-13 DOI:10.1016/j.actatropica.2024.107391
{"title":"Qualitative analysis of Fasciola gigantica excretory and secretory products coimmunoprecipitated with buffalo secondary infection sera shows dissimilar components from primary infection sera","authors":"","doi":"10.1016/j.actatropica.2024.107391","DOIUrl":null,"url":null,"abstract":"<div><p>Buffaloes cannot mount a robust adaptive immune response to secondary infection by <em>Fasciola gigantica</em>. Even if excretory and secretory products (ESPs) exhibit potent immunoregulatory effects during primary infection, research on ESPs in secondary infection is lacking, even though the ESP components that are excreted/secreted during secondary infection are unknown. Therefore, qualitative analysis of ESP during secondary infection was performed and compared with that of primary infection to deepen the recognition of secondary infection and facilitate immunoregulatory molecules screening. Buffaloes were divided into three groups: A (n = 3, noninfected), B (n = 3, primary infection) and C (n = 3, secondary infection). Buffaloes in the primary (0 weeks post infection; wpi) and secondary (-4 and 0 wpi) infection groups were infected with 250 metacercariae by oral administration. Then, sera were collected from groups at different wpi, and interacting proteins were precipitated by coimmunoprecipitation (Co-IP), qualitatively analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and annotated by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses to infer their potential functions. In group C, 324 proteins were identified, of which 76 proteins were consistently identified across 7 time points (1, 3, 6, 8, 10, 13, and 16 wpi). Compared with 87 proteins consistently identified in group B, 22 proteins were identified in group C. Meanwhile, 34 proteins were only identified in group C compared to 200 proteins identified in group B. Protein pathway analysis indicated that these proteins were mainly involved in the cellular processes and metabolism of <em>F. gigantica</em>. Among them, 14–3–3θ was consistently identified in group C and may be involved in various cellular processes and innate immune signalling pathways. Members of the HSP family were identified in both groups B and C and may function in both primary and secondary infection processes. The proteins discovered in the present study will help to deepen the understanding of the molecular interactions between <em>F. gigantica</em> and buffalo during secondary infection and facilitate the identification of new potential immunoregulatory molecules.</p></div>","PeriodicalId":7240,"journal":{"name":"Acta tropica","volume":null,"pages":null},"PeriodicalIF":2.1000,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Acta tropica","FirstCategoryId":"3","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0001706X24002730","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PARASITOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Buffaloes cannot mount a robust adaptive immune response to secondary infection by Fasciola gigantica. Even if excretory and secretory products (ESPs) exhibit potent immunoregulatory effects during primary infection, research on ESPs in secondary infection is lacking, even though the ESP components that are excreted/secreted during secondary infection are unknown. Therefore, qualitative analysis of ESP during secondary infection was performed and compared with that of primary infection to deepen the recognition of secondary infection and facilitate immunoregulatory molecules screening. Buffaloes were divided into three groups: A (n = 3, noninfected), B (n = 3, primary infection) and C (n = 3, secondary infection). Buffaloes in the primary (0 weeks post infection; wpi) and secondary (-4 and 0 wpi) infection groups were infected with 250 metacercariae by oral administration. Then, sera were collected from groups at different wpi, and interacting proteins were precipitated by coimmunoprecipitation (Co-IP), qualitatively analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS), and annotated by Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analyses to infer their potential functions. In group C, 324 proteins were identified, of which 76 proteins were consistently identified across 7 time points (1, 3, 6, 8, 10, 13, and 16 wpi). Compared with 87 proteins consistently identified in group B, 22 proteins were identified in group C. Meanwhile, 34 proteins were only identified in group C compared to 200 proteins identified in group B. Protein pathway analysis indicated that these proteins were mainly involved in the cellular processes and metabolism of F. gigantica. Among them, 14–3–3θ was consistently identified in group C and may be involved in various cellular processes and innate immune signalling pathways. Members of the HSP family were identified in both groups B and C and may function in both primary and secondary infection processes. The proteins discovered in the present study will help to deepen the understanding of the molecular interactions between F. gigantica and buffalo during secondary infection and facilitate the identification of new potential immunoregulatory molecules.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
与水牛继发感染血清共免疫沉淀的巨型法氏囊病菌排泄物和分泌物的定性分析显示出与原发感染血清不同的成分
水牛不能对巨型法氏囊病的二次感染做出强有力的适应性免疫反应。即使排泄物和分泌物(ESP)在原发感染时表现出了强大的免疫调节作用,但对继发感染时的ESP研究却十分缺乏,甚至连继发感染时排泄/分泌的ESP成分都不清楚。因此,我们对继发感染时的 ESP 进行了定性分析,并与原发感染时的 ESP 进行了比较,以加深对继发感染的认识,促进免疫调节分子的筛选。水牛被分为三组:A组(n = 3,未感染)、B组(n = 3,原发感染)和C组(n = 3,继发感染)。原发感染组(感染后 0 周;wpi)和继发感染组(-4 和 0 wpi)的水牛口服 250 个蛔虫。然后,收集各组在不同 wpi 期的血清,通过共免疫沉淀(Co-IP)沉淀相互作用的蛋白质,用液相色谱-串联质谱(LC-MS/MS)进行定性分析,并通过基因本体(GO)和京都基因和基因组百科全书(KEGG)分析进行注释,以推断其潜在功能。在 C 组中,共鉴定出 324 个蛋白质,其中 76 个蛋白质在 7 个时间点(1、3、6、8、10、13 和 16 wpi)上得到一致鉴定。蛋白质通路分析表明,这些蛋白质主要参与了千头蝇的细胞过程和新陈代谢。其中,14-3-3θ 始终在 C 组中被鉴定出来,可能参与了各种细胞过程和先天免疫信号通路。在 B 组和 C 组中都发现了 HSP 家族成员,它们可能在原发性和继发性感染过程中发挥作用。本研究中发现的蛋白质将有助于加深对巨尾蝇蛆与水牛在继发感染过程中的分子相互作用的理解,并有助于鉴定新的潜在免疫调节分子。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
Acta tropica
Acta tropica 医学-寄生虫学
CiteScore
5.40
自引率
11.10%
发文量
383
审稿时长
37 days
期刊介绍: Acta Tropica, is an international journal on infectious diseases that covers public health sciences and biomedical research with particular emphasis on topics relevant to human and animal health in the tropics and the subtropics.
期刊最新文献
Cutaneous myiasis by Calliphoridae dipterans in dogs from Chad. Surveillance of coronavirus in wild mammals seized and rescued by the National Forest and Wildlife Service of Peru. Detection of Theileria in cattle ticks (Rhipicephalus microplus) (Canestrini, 1888) in upper-northeastern Thailand Influence of metalloproteinase-3 (-1171 5A>6A) polymorphism on periportal fibrosis in patients with schistosomiasis mansoni, Pernambuco, Brazil. An immuno-inflammatory profiling of asymptomatic individuals in a malaria endemic area in Uganda.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1