{"title":"Dialdehyde cellulose-based mixed-mode stationary phases for diversified high-performance liquid chromatography separations","authors":"","doi":"10.1016/j.chroma.2024.465349","DOIUrl":null,"url":null,"abstract":"<div><p>To deal with complicated separation situations, this study successfully prepared two mixed-mode chromatography (MMC) stationary phases, CCL-SIL and PCL-SIL, by functionalizing dialdehyde cellulose (DAC) derivatives. In liquid chromatography applications, CCL-SIL exhibited superior separation performance for nucleosides and bases in HILIC mode, while PCL-SIL performed better in RPLC and IEC modes. Their distinct separation mechanisms were also elucidated by quantum chemical calculations. Both CCL-SIL and PCL-SIL showed good stability and reproducibility, with relative standard deviations of retention time, peak area, and peak height below 7.79 % and 4.37 % for multiple injections. Particularly, the PCL-SIL column and the CCL-SIL column were successfully used for the quantitative analysis of trace targets in real samples with complex matrix, demonstrating high accuracy and precision.</p></div>","PeriodicalId":347,"journal":{"name":"Journal of Chromatography A","volume":null,"pages":null},"PeriodicalIF":3.8000,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Chromatography A","FirstCategoryId":"1","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0021967324007234","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"BIOCHEMICAL RESEARCH METHODS","Score":null,"Total":0}
引用次数: 0
Abstract
To deal with complicated separation situations, this study successfully prepared two mixed-mode chromatography (MMC) stationary phases, CCL-SIL and PCL-SIL, by functionalizing dialdehyde cellulose (DAC) derivatives. In liquid chromatography applications, CCL-SIL exhibited superior separation performance for nucleosides and bases in HILIC mode, while PCL-SIL performed better in RPLC and IEC modes. Their distinct separation mechanisms were also elucidated by quantum chemical calculations. Both CCL-SIL and PCL-SIL showed good stability and reproducibility, with relative standard deviations of retention time, peak area, and peak height below 7.79 % and 4.37 % for multiple injections. Particularly, the PCL-SIL column and the CCL-SIL column were successfully used for the quantitative analysis of trace targets in real samples with complex matrix, demonstrating high accuracy and precision.
期刊介绍:
The Journal of Chromatography A provides a forum for the publication of original research and critical reviews on all aspects of fundamental and applied separation science. The scope of the journal includes chromatography and related techniques, electromigration techniques (e.g. electrophoresis, electrochromatography), hyphenated and other multi-dimensional techniques, sample preparation, and detection methods such as mass spectrometry. Contributions consist mainly of research papers dealing with the theory of separation methods, instrumental developments and analytical and preparative applications of general interest.