The use of metagenomic and untargeted metabolomics in the analysis of the effects of the Lycium barbarum glycopeptide on allergic airway inflammation induced by Artemesia annua pollen

IF 4.8 2区医学 Q1 CHEMISTRY, MEDICINAL Journal of ethnopharmacology Pub Date : 2024-09-11 DOI:10.1016/j.jep.2024.118816

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Abstract

Ethnopharmacological relevance

The prevalence of allergic airway inflammation (AAI) worldwide is high. Artemisia annua L. pollen is spread worldwide, and allergic diseases caused by its plant polysaccharides, which are closely related to the intestinal microbiota, have anti-inflammatory effects. Further isolation and purification of Lycium barbarum L. yielded its most effective component Lycium barbarum L. glycopeptide (LbGP), which can inhibit inflammation in animal models. However, its therapeutic effect on AAI and its mechanism of regulating the intestinal flora have not been fully investigated.

Aim of the study

To explore LbGP in APE-induced immunological mechanisms of AAI and the interaction mechanism of the intestinal flora and metabolites.

Methods

A mouse model of AAI generated from Artemisia annua pollen was constructed, and immunological indices related to the disease were examined. A combination of macrogenomic and metabolomic analyses was used to investigate the effects of LbGP on the gut microbial and metabolite profiles of mice with airway inflammation.

Results

LbGP effectively alleviated Artemisia. annua pollen extract (APE)-induced AAI, corrected Th1/Th2 immune dysregulation, decreased Th17 cells, increased Treg cells, and altered the composition and function of the intestinal microbiota. LbGP treatment increased the number of Odoribacter and Duncaniella in the intestines of the mice, but the numble of Alistipes and Ruminococcus decreased. Metabolite pathway enrichment analysis were used to determine the effects of taurine and hypotaurine metabolism, bile acid secretion, and pyrimidine metabolism pathways on disease.

Conclusion

Our results revealed significant changes in the macrogenome and metabolome following APE and LbGP intervention, revealed potential correlations between gut microbial species and metabolites, and highlighted the beneficial effects of LbGP on AAI through the modulation of the gut microbiome and host metabolism.

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利用元基因组学和非靶向代谢组学分析枸杞糖肽对黄花蒿花粉诱发的过敏性气道炎症的影响

民族药理学相关性全世界过敏性气道炎症（AAI）的发病率很高。黄花蒿花粉遍布全球，其植物多糖引起的过敏性疾病与肠道微生物群密切相关，具有抗炎作用。对枸杞的进一步分离和纯化得到了其最有效的成分枸杞糖肽（LbGP），它可以抑制动物模型的炎症反应。研究目的探讨枸杞多糖在 APE 诱导的 AAI 免疫学机制中的作用，以及肠道菌群与代谢产物的相互作用机制。方法构建了由黄花蒿花粉产生的 AAI 小鼠模型，并检测了与疾病相关的免疫学指标。结果 LbGP能有效缓解茵陈蒿花粉提取物（APE）诱导的AAI，纠正Th1/Th2免疫失调，减少Th17细胞，增加Treg细胞，并改变肠道微生物群的组成和功能。LbGP治疗增加了小鼠肠道中Odoribacter和Duncaniella的数量，但Alistipes和Ruminococcus的数量减少了。通过代谢物通路富集分析，确定了牛磺酸和低牛磺酸代谢、胆汁酸分泌和嘧啶代谢通路对疾病的影响。结论我们的研究结果表明，在 APE 和 LbGP 干预后，宏基因组和代谢组发生了显著变化，揭示了肠道微生物物种和代谢物之间的潜在相关性，并强调了 LbGP 通过调节肠道微生物组和宿主代谢对 AAI 的有益影响。

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来源期刊

Journal of ethnopharmacology 医学-全科医学与补充医学

CiteScore

10.30

自引率

5.60%

发文量

967

审稿时长

77 days

期刊介绍： The Journal of Ethnopharmacology is dedicated to the exchange of information and understandings about people''s use of plants, fungi, animals, microorganisms and minerals and their biological and pharmacological effects based on the principles established through international conventions. Early people confronted with illness and disease, discovered a wealth of useful therapeutic agents in the plant and animal kingdoms. The empirical knowledge of these medicinal substances and their toxic potential was passed on by oral tradition and sometimes recorded in herbals and other texts on materia medica. Many valuable drugs of today (e.g., atropine, ephedrine, tubocurarine, digoxin, reserpine) came into use through the study of indigenous remedies. Chemists continue to use plant-derived drugs (e.g., morphine, taxol, physostigmine, quinidine, emetine) as prototypes in their attempts to develop more effective and less toxic medicinals.